碩士 / 國立清華大學 / 生命科學系 / 91 / Ionotropic glutamate receptors (iGluRs) mediate excitatory synaptic transmission in the vertebrate CNS. Based on pharmacological and electrophysiological criteria, iGluRs have been traditionally classified into three major subtypes: AMPA, kainate, and NMDA receptors. The C-terminus of AMPA receptors is intracellular and is involved in receptor anchoring. In this study, we examined the expression patterns of GluR1a, GluR2a, and GluR2b C-terminal alternative splicing isoforms by RT-PCR. We found that during zebrafish development, the GluR1ac (the long C-terminus isoform) RNA is more abundant than GluR1a (the short C-terminus isoform) at all stages, and the ratio between GluR1a and GluR1ac increases with the development. The GluR2ac (the long C-terminus isoform) RNA is more abundant than GluR2a (the short C-terminus isoform) before 10 hpf, but the GluR2a RNA increases dramatically at 24 hpf and remains as the dominant form thereafter. There is no significant change of RNA expression between GluR2b (the short C-terminus isoform) and GluR2bc (the long C-terminus isoform) at all stages, where GluR2b is the major form. RT-PCR also reveals the existence of maternally transcribed glutamate receptor RNAs, but the amounts of these maternal RNAs decline to a non-detectable level by 8 to 10 hpf. The glutamate receptor RNAs levels then increase gradually after 10 hpf. GluR1a and GluR1b RNAs expression patterns differ at later developmental stages and so do GluR2a and GluR2b. These results show that the gene expression patterns of the paralogous genes differ, suggesting that the functions of these genes may be different.
| Identifer | oai:union.ndltd.org:TW/091NTHU0105061 |
| Date | January 2003 |
| Creators | Wu Chang-Hua, 吳長樺 |
| Contributors | 周姽嫄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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