碩士 / 國立海洋大學 / 水產養殖學系 / 91 / Abstract
This study was conducted to evaluate the effect of β-glucan and vitamin C (L-ascorbic acid, LAA) on non-specific immune response of white shrimp (Litopenaeus vannamei) by oral administration. We measured the immunological parameters such as the superoxide anion (O2-) production and the enzyme activities of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), phenoloxidase (PO) and superoxide dismutase (SOD).
The results showed that the enzyme activities of GOT and GPT in the haemolymph of the shrimps fed with diets containing different dose of β-glucan and vitamin C were significantly lower than control groups (p＜0.05). This result indicated that β-glucan and vitamin C were helpful to wound healing of white shrimp. A significant (p＜0.05) increase of intrahaemocytic O2- was generated in shrimps fed with diets containing different dose of β-glucan and vitamin C using nitroblue tetrazolium (NBT) reduction assay. Moreover, we found that β-glucan was more effective than vitamin C in stimulating the O2- production and increased O2- production was observed from 2-4 days. However, on day 8 the lowest O2- production was detected. The enzyme activity of phenoloxidase was significantly (p＜0.05) evaluated in shrimps fed with diets containing different dose of β-glucan and vitamin C. In addition, the greatest phenoloxidase activity occurred on 2-4 days, and decreased gradually on day 4. However, after day 4 β-glucan and vitamin C had no significantly effect on enhancing the phenoloxidase activity (p＞0.05). Those results showed that prolonged use of immunostimulants such as β-glucan and vitamin C may decrease the immunity of the shrimps. A significant (p＜0.05) increase of enzyme activity of superoxide dismutase was generated in shrimps fed with diets containing different dose of β-glucan and vitamin C from 4-8 days. However, in contrast with the O2- production, we found that increased O2- production was observed from 2-4 days.
The results also showed that the shrimps fed with 1 g/kg diet β-glucan was effective in enhancing O2- production and the enzyme activities of phenoloxidase and superoxide dismutase from 2-4 days. And the shrimps fed with 0.2g/kg diet vitamin C were helpful to wound healing and the enzyme activities decreased from day 1. Moreover, the shrimps had higher non-specific immune response when β-glucan was used together with vitamin C in the diets. But prolonged use of immunostimulants such as β-glucan and vitamin C may decrease the immunity of the shrimps. Consequently, on the basis of cost and effects, we may feed white shrimp with diets containing β-glucan and vitamin C for 4 days with an intermission of a few days. This way may maximize the effectiveness of enhancing the non-specific immune response of white shrimps and reduce the amount of dietary β-glucan and vitamin C.
| Identifer | oai:union.ndltd.org:TW/091NTOU0086019 |
| Date | January 2003 |
| Creators | 廖述育 |
| Contributors | 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 104 |
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