碩士 / 國立海洋大學 / 水產養殖學系 / 91 / Marsupenaeus japonicus (6.37 ± 1.29 g) individually exposed to nine different combined environments of ammonia-N and nitrite-N {ammonia-N at 0.04 (control), 5.40 and 20.84 mg/l combined with nitrite-N at 0.02 (control), 5.29 and 20.80 mg/l } in 30 ppt (parts per thousand) seawater were examined for nitrogen excretions, accumulations of ammonia, nitrite, urea and uric acid in tissues, and hemolymph osmolality, electrolyte, oxyhemocyanin, protein and acid-base balance after 48 h. M. japonicus exposed to 5.40 mg/l ammonia-N - 5.29 mg/l nitrite-N increased its excretions in urea-N and organic-N by a factor of 2.2 and 5.7, respectively as compared to the shrimp only exposed to 5.40 mg/l ammonia-N. M. japonicus exposed to 5.40 mg/l ammonia-N - 5.29 mg/l nitrite-N decreased its hemolymph uric acid, gill ammonia, gill urea, gill uric acid, hepatopancreas ammonia, hepatopancreas urea and hepatopancreas uric acid by 37 %, 24 %, 6 %, 38 %, 18 %, 11 % and 14 %, respectively as compared to the shrimp only exposed to 5.40 mg/l ammonia-N.
M. japonicus exposed to 5.40 mg/l ammonia-N - 5.29 mg/l nitrite-N decreased its hemolymph osmolality and decreased its hemolymph Na+ and Cl- by a factor of 9 and 8 %, respectively as compared to the shrimp only exposed to 5.40 mg/l ammonia-N.
M. japonicus exposed to 5.40 mg/l ammonia-N - 5.29 mg/l nitrite-N decreased its hemolymph oxyhemocyanin and protein by 13 and 6 %, as compared to the shrimp only exposed to 5.40 mg/l ammonia-N. M. japonicus exposed to 5.40 mg/l ammonia-N - 5.29 mg/l nitrite-N decreased its hemolymph pH, PCO2, HCO3-, TCO2 and OH-/H+ by 0.7 %, 22 %, 24 %, 24 %, and 7 %, as compared to the shrimp only exposed to 5.40 mg/l ammonia-N. M. japonicus exposed to ambient 5.40 mg/l ammonia-N - 5.29 mg/l nitrite-N increased its hemolymph PO2 by a factor of 1.4 as compared to the shrimp only exposed to 5.40 mg/l ammonia-N.
It is concluded that M. japonicus exposed to elevated ambient ammonia-N in the presence of ambient nitrite-N displayed an increase in nitrogen metabolism, productions of urea-N, organic-N, and hemolymph PO2, but decreased in hemolymph osmolality, electrolyte concentration ions, oxyhemocyanin, hemolymph protein, pH, PCO2, HCO3-, TCO2 and. That is a synergistic effects on nitrogen metabolism, accumulation of nitrogen and acid-base balance for the shrimp following exposure to combined environments of ammonia-N and nitrite-N.
| Identifer | oai:union.ndltd.org:TW/091NTOU0086029 |
| Date | January 2003 |
| Creators | Hsieh Li-Wei, 謝立偉 |
| Contributors | Chen Rong-Xiang, 陳榮祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 124 |
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