碩士 / 國立海洋大學 / 水產養殖學系 / 91 / This study examined the immune response of the white shrimp Litopenaeus vannamei, and its resistance against Vibrio alginolyticus when the shrimp were injected with sodium alginate and laminarin.
L. vannamei which injected with sodium alginate at doses of 10, 20 and 50μg g-1 were examined for the immune parameters after 0, 1, 2, 4 and 6 d. No significant differences in total haemocyte count, differential haemocyte count and superoxide dismutase activity were observed among the shrimp injected with sodium alginate at 10, 20, 50μg g-1, saline and the control shrimp. L. vannamei injected with sodium alginate at a dose of 20μg g-1 increased its phenoloxidase activity and respiratory burst after 2 d. L. vannamei injected with 10μg g-1 increased its phagocytic activity and clearance efficiency to V. alginolyticus after 1 d. In other experiment, L. vannamei which had been injected with sodium alginate were challenged with V. alginolyticus at 2×105 colony-forming units(cfu) per shrimp and then placed in seawater(34 ppt). Survival of shrimp injected with sodium alginate at a dose of 50μg g-1 at 2 d post-injection, and the survival of shrimp injected with sodium alginate at a dose of 10μg g-1 at 4 d post-injection increased significantly as compared to the shrimp injected with saline and the control shrimp. It is concluded that sodium alginate at a dose of 10μg g-1 or higher causes an increases in the immune response of L. vannamei and resistance from V. alginolyticus infection.
No significant differences in total haemocyte count, phenoloxidase, superoxide anion and phagocytic activity were observed among the shrimp injected with saline and those injected with laminarin at doses of 10, 20 and 50μg g-1. L. vannamei injected with laminarin at 50μg g-1 increased its superoxide dismutase activity after 1 d. In other experiment, L. vannamei which had been injected with laminarin were challenged with V. alginolyticus at 2×105 cfu per shrimp, and then placed in seawater（34 ppt）. Survival of shrimp injected with laminarin at a dose of 50μg g-1 at 2 d post-injection increased significantly as compared to the shrimp injected with saline and the control shrimp. L. vannamei injected with laminarin at 50μg g-1 increased its clearance efficiency after 1 d.
This study indicated that both sodium alginate and laminarin can enhance the immune ability of L. vannamei. A continuous dose is necessary to maintain the immune ability of shrimp. Further research is needed to evaluate the immune stimulatory effect of L. vannamei and its resistance against V. alginolyticus when shrimp are immersed in seawater containing sodium alginate or laminarin, or fed with sodium alginate or laminarin containing diets.
| Identifer | oai:union.ndltd.org:TW/091NTOU0086032 |
| Date | January 2003 |
| Creators | YEH SU-TUEN, 葉舒屯 |
| Contributors | CHEN JIANN-CHU, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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