碩士 / 國立海洋大學 / 水產養殖學系 / 91 / The catfish, Clarias fuscus, is an economical and local species in Taiwan. However, the land was developed excessively to cause enormous destruction for habitat. In order to raise economic effect, hence introduce C. batrachus and C. mossambicus before and after. But foreign species was not accepted with consumer and discarded. Cause the strain disorder and hybrid of the catfish. Therefore, use RAPD to eliminate cryptic hybrid from germplasm.
RAPD markers assays are based on the amplification by polymrase chain reaction (PCR) of random DNA using primer consisting of small inverted repeats of artitrary oligonucleotid sequences. We tested 200 RAPD primers from UBC (University of British Columbia) for the genetic polymorphism in catfish. Of the 200 primers, 16 were good, but just only 8 primers had clean, stable and reproducible RAPD pattern. Use primers # 211, 245, 287 make specific and general probe to hybridization the PCR product and genomic DNA. The result of only C. mossambicus and Minder Dam sample can hybridize by specific probe. Demonstrate the Minder Dam sample probably the offspring of C. mossambicus cross C. fuscus.
In addition, the RAPD marker can descend to offspring from parent. Therefore, use specific probe to detect the hybrid strain of C. mossambicus cross C. fuscus. The result of all hybrid strain can hybridize by specific probe.
| Identifer | oai:union.ndltd.org:TW/091NTOU0086037 |
| Date | January 2003 |
| Creators | Yu-Hsuan Lin, 林育萱 |
| Contributors | Jau-Der Chen, 陳昭德 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 118 |
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