碩士 / 國立海洋大學 / 食品科學系 / 91 / The study was aimed at quantifying the optimal vitamin K (menadione sodium bisulfite, MSB) requirements of juvenile hybrid tilapia (Oreochromis niloticus ´ O. aureus) and grouper (Epinephilus malabaricus). In Experiment I, purified diets with 8 levels (0, 0.2, 0.5, 1, 2, 5, 10, 20 mg MSB/kg) of supplemental vitamin K were each fed to triplicate groups of tilapia (mean initial weight 0.52 ± 0.01 g) for 8 weeks. Results indicated that weight gain (WG) was higher (P<0.05) in fish fed diet supplemented with 5 mg MSB/kg than fish fed diets with £2 and ³10 mg MSB/kg. Feed efficiency (FE) was higher in fish fed diets supplemented with 2 and 5 mg MSB/kg than fish fed diets with 0.5, 1, 20 mg MSB/kg and unsupplemented control diet. Protein efficiency ratio (PER) was higher in fish fed diets supplememted with 2 and 5 mg MSB/kg than fish fed diets with £1 and ³10 mg MSB/kg. Plasma total prothrombin concentration and hepatic menaquinone-4 (MK-4) levels were higher in fish fed diets with ³5 mg MSB/kg than fish fed diets with £2 mg MSB/kg. Analysis by polynomial regression of WG, FE and by broken-line regression of plasma total prothrombin concentration indicate that the optimal dietary vitamin K requirement for tilapia is 5.2 mg/kg diet. In Experiment II, purified diets with 8 levels (0, 1, 2, 5, 8, 12, 15, 20 mg MSB/kg diet) of supplemental vitamin K were each fed to triplicate groups of grouper (mean initial weight 9.95 ± 0.05 g) for 10 weeks. Results indicated that vitamin K supplementation did not have significant effects (P>0.05) on WG, FE and PER in fish. Plasma total prothrombin concentration was higher in fish fed diets with ³8 mg MSB/kg than fish fed diets with £5 mg MSB/kg. Hepatic MK-4 levels was higher in fish fed diets supplemented with ³15 mg MSB/kg than fish fed diets with £12 mg MSB/kg. Analysis by broken-line regression of the plasma total prothrombin concentration indicate that the optimal dietary vitamin K requirement for grouper is 10.6 mg/kg diet.
| Identifer | oai:union.ndltd.org:TW/091NTOU0253042 |
| Date | January 2003 |
| Creators | 李貞儀 |
| Contributors | 蕭錫延 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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