碩士 / 國立海洋大學 / 食品科學系 / 91 / Micrococcus sp. strain DB05B was isolated from the biofouling material suspended in sea water. While 5% lactose and 0.71% yeast extract were used to replacing the carbon source and nitrogen source of mucous screening basal broth (MSBB), the results indicated that the extracellular adhesive substance (EAS) yields were increased from 0.29-0.31 g/L up to 0.74 g/L and 0.65 g/L, respectively. Ten ppm Mn2+ metal or 1.5 mM disodium-β-glycerophosphate (DSGP) was added into the MSBB individually, and then higher EAS yields, 0.57 and 0.68 g/L were achieved, respectively. Modified-mucous production broth-lactose (M-MPB-L) is composed of the individual best cultured medium ingredient for strain DB05B. The effect of culture conditions were examined individual, the results showed that while the initial pH at 7.2, incubation temperature at 26oC, and shaking speed with 150 rpm, the highest EAS yields of 0.80-0.85 g/L could be reached in each condition tested. The effect of different substrata, such as activated carbon flake, chitin flake, wood piece, stainless steel beads, glass beads, or polypropylene beads, on the EAS yield produced from strain DB05B were also investigated. The chitin flake added to M-MPB-L performed the highest EAS yield, as 0.89 ± 0.02 g/L, as compared to other testing substrata, the second for EAS yield was activated carbon flake, which obtained 0.77 ± 0.21 g/L. The compositions of EAS lyophilized powders that obtained from strain DB05B culturing with the MSBB or M-MPB-L had moisture content as 10.8% and 10.9%, carbohydrate content as 60.8% and 63.4%, and protein content as 14.8% and 18.3%, respectively. The relative viscosity (RV) of EAS rehydrated aqueous solution was increased while the concentration of EAS was increased in the reacting solution. However, EAS solution with the addition of increasing NaCl concentration from 0.0%-5.0% were performed lower RV. The RV of 0.2% lyophilized EAS powder rehydrated solution was increasing while reacting temperature (10-80oC) were decreased. The 0.2% lyophilized EAS powder rehydrated solution showed the higher RV while the pH of reacting solution was adjusted to 2.0 than it did in other adjusted pH value (3.0-10.0). Effect of EAS addition on emulsifying activity and emulsion stability while mixed with isolated soy protein, the result showed that emulsifying activities of 0.0-1.0% EAS were ranged from 37% to 42%, and their emulsion stabilities were ranged from 21% to 40%. The 1.0-2.0% EAS rehydrated solution performed the capability of film formation. The EAS recovered from the cultivation of strain DB05B in MSBB or M-MPB-L were used to test for their capability on antioxidation in the concentration ranged from 5 to 15 mg/mL. The ability for inhibition of hemoglobin catalyzing linoleic acid was examined, the lyophilized EAS derived from MSBB showed a better result than the EAS derived from M-MPB-L. While the ability for scavenging DPPH free radical was examined, the lyophilized EAS obtained from MSBB (15 mg/mL) or M-MPB-L (15 mg/mL) performed antioxidative activity as 6.70 ± 0.01% and 3.56 ± 0.00%, respectively. For evaluating their antioxidation effect, lyophilized EAS obtained from MSBB (15 mg/mL) or M-MPB-L (15 mg/mL) presented 93.46 ± 0.08% and 56.33 ± 1.11% capability on the chelating Fe2+ ion, respectively.
| Identifer | oai:union.ndltd.org:TW/091NTOU0253049 |
| Date | January 2003 |
| Creators | Ya Chen Li, 李亞蒨 |
| Contributors | Chorng Liang Pan, 潘崇良 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 76 |
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