碩士 / 國立海洋大學 / 環境生物與漁業科學學系 / 91 / Abstract
The ecosystems of coral reefs have an unique life phenomenon. It is called“endosymbiosis”. We tried to study endosymbiosis using cellular and molecular biology techniques. Rab GTP-binding proteins regulate vesicular trafficking consisting of vesicle budding, fusion, and transport. We have cloned and characterized a novel Aiptasia pulchella Rab39(ApRab39) cDNA. The ApRab39 cDNA is 1600bp in length and encodes a protein of 240 amino acids with a 56% identity to the human Rab39B. ApRab39 contains consensus sequences involved in GDP/GTP binding and protein prenylation. By transfection of CHO and HeLa cell with a plasmid encoding EGFP-ApRab39, we found that EGFP- ApRab39 was localized mainly in cytosol. However, 36 hours after transfection, EGFP-ApRab39 localization displayed a peri-nuclear pattern, reminiscent of ER or ER associated organelles. By immunstaining, ApRab39 was found to be associated with symbiosomes in Aiptasia pulchella and therefore, may be a symbiosis associated protein. Overall, these results suggest that ApRab39 plays an important role in the endosymbiosis between Aiptasis pulchella and its algal symbionts.
| Identifer | oai:union.ndltd.org:TW/091NTOU0451016 |
| Date | January 2003 |
| Creators | HUANG HSING YEN, 黃倖妍 |
| Contributors | LEE KUO TIEN, FANG LI HSING, CHEN MING CHUAN, 李國添, 方力行, 陳鳴泉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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