碩士 / 國立海洋大學 / 生物科技研究所 / 91 / The arsenic species in oyster (Crassostrea gigas) from different regions and seasons obtained in west coast, Taiwan are investigated in this research. The arsenic species studies include TAsdm (total arsenic based on dry mass), TAsex (total extractable arsenic), TAsresidue (total residual arsenic), TAsws (total water soluble arsenic), TAsls (total lipid soluble arsenic), iAs (total inorganic arsenic), MMA (monomethylarsonic acid), DMA (dimethylarsinic acid), AsB (arsenobetaine) and others (portion of non analyzed portion in water soluble aliquot). The established methods of all different arsenic species are optimized separately in the initial stage. Afterwards, both seasonal and regional variations of different arsenic species were inspected. The health impact on consumption of the oysters form west coast were also evaluated in this study.
In the DORM-2 (NRCC dogfish muscle certified reference material) analysis according to the TAsdm and AsB methods, both recovery rate of the reference value were 98%. The recovery rate of analyzing oyster tissue standard reference material of NIST 1566-b by TAsdm was 105%. The result of studying iAs in DORM-2 was similar to the findings of other literature studies. All the research methods were extensively optimized and quality control were performed on oyster analytical procedure, thus has made the MMA and DMA results fairly accurate.
We found that according to the TAsdm analysis, oysters in winter had higher TAsdm, which was 14.48 mg/kg, but only 11.58 mg/kg in summer in average. As to different regions, Hsiangshan had the highest concentration that was 15.18 mg/kg; but that of Budai was only 12.63 mg/kg. According to the ANOVA results, there was no statistic difference between any two regions, however.
The average arsenic species concentrations were: iAs 1 %,
MMA 0.4 %, DMA 4.4%, and AsB 47.1%. As the dietary allowance of iAs for a 60 kg adult is 900 mg per week, and input the average iAs content in oysters in west coast 0.15 mg/kg as well as the average water content 84%, an adult has to consume more than 37 kg of oysters to cause the health impact.
| Identifer | oai:union.ndltd.org:TW/091NTOU0613002 |
| Date | January 2003 |
| Creators | Chia-Wei Huang, 黃佳偉 |
| Contributors | Tung-Ming Hsiung, 熊同銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 132 |
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