碩士 / 國立台北師範學院 / 數理教育研究所 / 91 / Diseased cobias were collected from some net-cage farms at Hsiqu Liouchiou Island, next to Dunggang, Taiwan. Some little nodes appeared on surfaces of their fins and skins. When the symptom became serious, they looked like sarcomas. Almost every organ, including liver, spleen, kidney, fin, heart, stomach, intestine of a hard-sick cobia were dealt through the following processes：fixation, section and staining. Only at the connective tissues of fins and epithelium did we find hypertrophy cells which own big nuclei and inclusion bodies around the nuclei. Owing to the piles of the cells, some nodes appeared on fins and some places of epithelium.We tried to isolate the pathogenic organisms and infect the cells primarily cultured from cobia fins, named CF. The CF cell line was well cultured and passaged every 3~4 days. It had been passaged for over 30 times and owned three types─fibroblast like, little-fibroblast like and epithelium like. To get the opposite of what one wants, the CF failed to get infected with apparent CPE but changed it shape. So that we forgave making use of the CF to proliferate the virus in order to provide our research. As the ways to detect the virus, we compared Nested-PCR with DIG-labelled dot blot hybridization and hoped to rise their degree of accuracy, sensitivity and convenience. The answer was that the former could be far sensitive than the latter, but the former needed complex instruments. The latter could help us to directly judge the order of severity by colors.On the other hand, we took advantage of polymerase chain reaction（PCR）to amplify the MCP genome of the virus and compare it with other nine published MCP genomes of viruses including CzIV, TIV, CIV, LCDV-1, LCDV-K1, BIV, FV3, GIV, RSIV. The sequencing data showed that the MCP of Cobia iridovirus（CbIV）was more homologous to the MCP of LCDV-(K)1（89﹪similarity）than that from FV3（47﹪similarity）.
| Identifer | oai:union.ndltd.org:TW/091NTPTC476018 |
| Date | January 2003 |
| Creators | Wu Ren-Yu, 吳仁佑 |
| Contributors | , 施秀惠, 盧秀琴 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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