碩士 / 國立臺灣大學 / 畜產學研究所 / 91 / 英 文 摘 要
Effects of dietary algal docosahexaenoic acid supplement on laying Tsaiya ducks and Leghorn hens
Chao-hung Cheng
The purpose of this study was to study the effects of dietary docosahexaenoic acid (DHA) on yolk DHA content, and on the expressions of liver lipogenesis-related genes in order to alleviate the negative influence of liver fat deposition on egg laying performance.
There were three treatments with 10 Leghorn hens and 10 Tsaiya ducks per treatment. Diets were based on corn-soybean meal ( ME : 2803 Kcal/kg；CP : 17.1%；Ca : 3.4% ) with supplement of 0%, 0.5% or 2% DHA oil. After feeding control diet for one week, the birds were fed with the experimental diets for fourteen days. Results indicated that the egg production of three treatments of both birds was not affected by dietary DHA oil ( P>0.05 ). The egg weight and yolk size were not affected by the diets ( P>0.05 ). The DHA content in yolks of both laying birds increased with the supplementation of DHA significantly ( P<0.01 ). The DHA content of the egg yolk of Tsaiya ducks was 0.53%, 1.29% and 3.42% for 0%, 0.5% and 2% DHA treatments, respectively for 1st week, and 0.50%, 1.53% and 3.26% for 2nd week. In Leghorn hens, the yolk DHA content was 0.57%, 1.81% and 3.07% for the one-week treatments, and 0.57%, 1.81% and 3.07% for two-week treatments. Therefore, algal DHA oil can be utilized by Laying Tsaiya ducks and Leghorn hens to enhance egg yolk DHA content and produce high DHA eggs.
The concentratron of triacylglycerol and cholesterol in plasma of two birds were also measured. The data showed that they were not affected by dietary DHA ( P>0.05 ). The DHA content in the livers of Tsaiya ducks differed significantly among treatments ( P<0.05 ). They are 1.43%, 4.43%, and 10.13%, respectively in the livers of Tsaiya ducks treated with 0%, 0.5%, and 2% dietary DHA ; 1.92%, 5.90%, and 8.54% respectively in the livers of Leghorn hens. The DHA content of the muscle of Tsaiya ducks treated with 0%, 0.5%, and 2% dietary DHA was o.83%, 1.56%, and 1.95%, respectively and 0.38%, 0.86%, and 2.07% for the muscle of Leghorn hens. The data indicate that dietary DHA oil can be incorporated into egg yolks and tissues. The plasma DHA level was also affected by dietary supplementation ( P<0.05 )
The lipogenesis-related genes in the livers of Tsaiya ducks, SREBP1, SREBP2, FAS, and HMGCoA reductase were cloned and sequenced to generate probes for Northern analysis of these genes. These genes were not affected by the supplementation of 2% DHA oil for two weeks.
| Identifer | oai:union.ndltd.org:TW/091NTU00289004 |
| Date | January 2003 |
| Creators | Chao-Hung Cheng, 鄭兆宏 |
| Contributors | Shih-Torng Ding, Ph. D., 丁詩同 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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