碩士 / 國立臺灣大學 / 動物學研究所 / 91 / Heavy metals, Zn, Cd, Hg and Cu, are the major contaminants in ocean. They are released by industries and then washed by rain and finally flow into ocean. Abalone (Haliotis diversicolor ), an economically important mollusc living in coastal waters, have a great possibility to expose on those heavy metals. When heavy metals get into its hemolymph, the physiological hemocytes might be affected. The purpose of this research is to investigate how heavy metals affect hemocytes in abalone.
When exposed to heavy metals, 0.01mM Hg(II) and 1mM Cu(II) significantly affected mortality, adhesion and phagocytosis of abalone hemocytes hemocytes, but Zn(II) and Cd(II) didn’t. Antioxidants, 10mM vitamin C and 100μM vitamin E can decrease the toxic effects of 0.1mM Hg(II), but not Cu(II). Furthermore, vitamins enhance the toxic effects of 8mM Cu(II). On the other hand, the results of lipid peroxidation showed that 1mM Hg(II) and 8mM Cu(II) increased oxidative stress in abalone hemocytes. Also, lipid peroxidation was decreased when vitamin E or vitamin C presented. The toxic effects of Cu(II) is not only from oxidative stress, and its toxic effects was not overcomed by vitamin C or vitamin E, so this could be investigated in the future.
Identifer | oai:union.ndltd.org:TW/091NTU00312017 |
Date | January 2003 |
Creators | Chih-Hao Chang, 張智豪 |
Contributors | Jiun-Hong Chen, 陳俊宏 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 42 |
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