碩士 / 國立臺灣大學 / 漁業科學研究所 / 91 / The purpose of the present study is to explore the relation between osmoregulation and HSP90 in tilapia. Effects of +10C heat shock and transfer to 20 ppt SW on the expression of gill HSP90 and Na+, K+-ATPase as well as plasma osmolarilty and body Na+ content in tilapia (Oreochromis mossambicus) were examined. Moreover, specific inhibitor of HSP90, geldanamycin, was used to reconfirm the relationship between HSP90 and osmoregulation in tilapia during heat shock and SW acclimation. The protein expression of HSP90 and Na+, K+-ATPase in heat shock group was higher than that in control group, and the value of osmolarity and Na+ concentration in heat shock group was lower than that in control group. The rapid heat shock treatment is helpful for heat shock group stimulating Na+, K+-ATPase and consequently, enhancing salinity acclimation. Immersion of the specific inhibitor of HSP90, geldanamycin, caused tilapia to show a significantly higher Na+ content than the control after heat shock and SW transfer. Based on these data, we concluded that HSP90 was influential to the osmoregulation mechanisms in fish during SW acclimation.
| Identifer | oai:union.ndltd.org:TW/091NTU00451020 |
| Date | January 2003 |
| Creators | Wu, Hong-chih, 吳鴻志 |
| Contributors | Hwang, Pung-pung, 黃鵬鵬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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