碩士 / 國立臺灣大學 / 獸醫學研究所 / 91 / Three species of streptococci(Enterococcus faecalis, Streptococcus iniae, and Lactococcus garvieae) isolated from aquacultured fish of Taiwan were identified by 16s rRNA sequencing. The formalin-killed whole cell vaccines of theses streptococci were prepared. Mullet (Mugil cephalus), and tilapia (Oreochromis spp.) were vaccinated by hyperosmotic immersion to evaluate the RPS of this vaccine. Besides, macrophage and sera of vaccinated fish were evaluated including bactericidal activity and Ab titer. In mullet and tilapia, the RPS was 58.3-73.2 and 71.2-88.7 respective, and. the antibody titers could be detected at 2nd week, and ascended obviously at 5th to 6th week after vaccinated. At 1st week after vaccinated to tilapia, bactericidal activity of sera did not increase, but the bactericidal activity of macrophages increased. There is no significant difference of protection between single vaccination and booster vaccination. These formalin-killed vaccines of streptococci elicited a protective response to clear off the invasive streptococci in fishes during early weeks after vaccinated, even though the antibody titers were low, the macrophages should play a more important role of protection from strepotococcosis in fish than anti-sera do.
| Identifer | oai:union.ndltd.org:TW/091NTU00541011 |
| Date | January 2003 |
| Creators | Chen-Hsi Chung, 鐘禛熙 |
| Contributors | Meei-Mei Chen, 陳媺玫 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 62 |
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