碩士 / 國立臺灣大學 / 醫學工程學研究所 / 91 / Abstract
The mechanism whereby the cell synthesizes and secrets an
extra-cellular matrix and then in turn is regulated by it, is termed dynamic reciprocity. The present work aimed to fabricate tri-copolymer from gelatin,hyaluronic acid and chondoitin-6-sulfate to mimic natural cartilage matrix as scaffold for cartilage tissue engineering. The scaffold has uniform pore size about 300-500μm in average. In this study the tri-copolymer scaffolds were seeded with porcine chondrocytes and cultured spinner flask for 2, 4, and 6 weeks. Chondrocytes were homogenously distributed in scaffold when cultured in spinner flask. Secretion of extra-cellular matrix was found under histology examination by H&E, Alcian-blue, type II collagen antibody, S-100 protein to prove chondrocyte keep its phenotype at least for 2 weeks under the culture system. The results were also proven by the RT-PCR on the expression of type I collagen, type II collagen and type X collagen. The results showed that the developed tri-copolymer scaffold grafted with TGF-β1 had a better condition for articular cartilage growth in vitro. The scaffold was then implanted into the porcine articular cartilage with a defect of 3 mm in diameter. The results were compared with the mosaicplasty. The results of the animal study were evaluated by the H&E, Alcian blue, type II collagen antibody, S-100 protein staining. The results indicate gelatin- hyaluronic acid- chondroitin-6-sulfate tri-copolymer is a promising material as scaffold for
cartilage tissue engineering.
| Identifer | oai:union.ndltd.org:TW/091NTU01530002 |
| Date | January 2003 |
| Creators | Cheng-Hung Chou, 周正鴻 |
| Contributors | Feng-Huei Lin, Jui-Sheng Sun, 林峰輝, 孫瑞昇 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 103 |
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