碩士 / 國立雲林科技大學 / 環境與安全工程系碩士班 / 91 / This study focuses to promote the capability of EDTA degradation of Burkhol cepacia YL-6 bacterium, EDTA degradation which was isolated from the environment of Taiwan, we try to add photocatalyst TiO2 to activate EDTA photodegradation or Compound E as carbon source to stimulate the growth of YL-6.
Cell concentration still keep the same about 250 mg/L, even TiO2 adding, there is no dramatically promote the cell growth. The result reveals that TiO2 can’t proceed photocatalytic reaction because the energy of lamplight is smaller than the requirement of photocatalytic RX.
To substitute EDTA with Compound E (1000mg/L) as a carbon source, the cell concentration can up to 1300 mg/L after 2 days incubation. It is obvious that Compound E as carbon source can promote cell growth five-fold, from 250mg/L to 1300mg/L. The study shows that using Compound E as a carbon source can increase the concentration of YL-6 effectively. The efficiency of degrading Fe-EDTA and Cu-EDTA are 94% and 82%. YL-6 strain was cultivated in the fermentor with compound E as carbon source under optimal condition, after 96 hours incubation, the maximun cell concentration reach to 3.6 g/L and possess a viable count of 2.6 ×1010 CFU/mL, then the broth mixed with wheat bran, and drying with warm wind under 35℃for 48 hours, and bioaugmentation agent was prepared for wastewater treatment, the bioaugmentation agents possess a viable count of 6.5 ×1010/g product. The degradation efficiency of bioaugmentation agent to Fe-EDTA and Cu-EDTA can reach 100% at 6 and 7days.
The characteristic properties from a PC board plants in Tao-Yuan, pH values is about 10.61, TOC 8750 mg/L, EDTA1.01mM. The wastewater is diluted and adjust pH value to 7.0. Then add Na2S to remove CuS(s), The color of wastewater turns from blue to transparent. Put 5 % YL-6 seed into 100 mL wastewater medium, YL-6 grow in wastewater with a little inhibation. The removal of EDTA and TOC are 62%, 50 %. The primary test reveals YL-6 can decontaminate TOC and EDTA from the wastewater of the PC board factory. Further study is keep going.
| Identifer | oai:union.ndltd.org:TW/091YUNT5633093 |
| Date | January 2003 |
| Creators | Kuo-hsu Li, 李國旭 |
| Contributors | Hung-yuan Fang, 方鴻源 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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