碩士 / 國立中興大學 / 食品科學系 / 92 / Heterotrophic culture has been one of the effective ways to increase
the yield and productivity of microalgae. In this study,
Chlorella pyrenoidosa NCHU-6, a useful green algal strain with
heterotrophic growth function and high yield, was used. At first,
optimization of cultivation conditions in shaking flasks in the absence of light was studied so that the results obtained may offer the reference for the subsequent determination of semi-continuous cultivation condition of C. pyrenoidosa by using fermentor without illumination to improve its
cellular chlorophyll content and crude protein.
Growing of C. pyrenoidosa NCHU-6 in shaking flasks at
30℃, 100 rpm and pH 6.5 for 5 days showed that fructose is the best
carbon source. The packed cell volume (PCV) and cellular chlorophyll
content were 50 ml/L and 1 mg/ml, respectively. And the PCV and
cellular chlorophyll content of algae by using glucose and lactose
as carbon source were a little lower.
The optimal glucose concentration for the production of C. pyrenoidosa
NCHU-6 was 4 %, which led to the highest packed cell volume
and cellular chlorophyll content, Besides, the average amount of glucose
consumed by C. pyrenoidosa NCHU-6 when one ml of algal PCVproduced was
lower.
As glucose is the only carbon source, the results showed that urea
was the optimal nitrogen source for C. pyrenoidosa NCHU-6 with the
packed cell volume being 30ml/L and cellular chlorophyll content being 0.84 mg/ml.
The optimal urea concentration for the production of C. pyrenoidosa
NCHU-6 was 10.672 g/L. And the highest packed cell volume
and cellular chlorophyll content were 0.83 mg/L and 50 mL/L,
respectively.However, combination of urea and KNO3 as nitrogen sources proved to be even better than either one used. The best packed cell volume and cellular chlorophyll content were 1.03mg/L and 50ml/L, respectively.Supplementing with organic nitrogen did not increase the algal growth and cellular chlorophyll content.
Finally, the optimal cultivation conditions in shakig flasks for
C. pyrenoidosa NCHU-6 were 28℃, initial pH 6.0 and shaking rate 110rpm. And the packed cell volume, algal chlorophyll content, crudeprotein content and CGF content were 57 mL/L, 32.34 mg/100 g, 65.52%,
1.15A260/mg, respectively.
After taking the detemined optimal cultivation conditions in shaking
flasks for the semi-continuous cultivation of C. pyrenoidosa NCHU-6 in
a 7L-fermentor, it was found that if 60%(v/v) of culture broth was
withdrawn and replaced with fresh medium in the middle of
cultivation, the algal chlorophyll content, crude protein content
and CGF content were raised to 35.85 mg/100 g, 66.20% and 1.42 A260/g.
Taking 40 %(v/v) of initial culture broth as starter, and the
feeding medium containing 6% glucose concentration was used for algal
cultivation with semi- continuous cultivation mode. Algal chlorophyll
content, and CGF content were raised to 39.90 mg/mL, 1.4 A260/g , and
the same crude protein content.The overall performance was superior to
those of batch culture.
| Identifer | oai:union.ndltd.org:TW/092NCHU0253039 |
| Date | January 2004 |
| Creators | Yu-Yuan Chia, 賈有元 |
| Contributors | Chin-Shun Chen, Ching-Sen Sheu, 陳錦樹, 許清森 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 126 |
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