碩士 / 國立中興大學 / 植物病理學系 / 92 / Abstract
Two virus cultures were isolated from leaves of calla lily (Zantedeschia spp.) (Zan-12B) and carnation (Dianthus caryophyllus L.) (CarMV-C) showing viral disease-like symptoms of yellow necrotic spots and yellow mottle on leaves in the fields in central Taiwan. Isometric particles about 32-35 nm in diameter were found in crude saps of diseased tissues from calla lily and carnation under electron microscopic examination. Similar particles were also observed in the cytoplasms of the mesophyll cells in ultrathin sections prepared from infected D. caryophyllus, D. chinensis and Zantedeschia spp.. SDS-polyacrylamide gel electrophoresis showed that the two viral isolates each contains a structural polypeptide about 40 kDa. Serological tests with double immunodiffusion test and double antibody sandwich-enzyme-linked immunosorbent assay showed that the two isolates reacted positively with an antiserum prepared against Carnation mottle virus (CarMV). Using primers specific to coat protein (CP) gene of CarMV, an expected viral CP gene product of 1.05 kb was amplified by reverse transcriptase polymerase chain reaction from total RNA isolated either from CarMV-C-infected C. quinoa or CarMV Zan12B-infected N. benthamiana. Comparisons of the 1047-nucleotide CP genes form CarMV-C and CarMV Zan12B with those of 24 CarMV isolates available in GenBank showed that the calla lily isolate (Zan-12B) had 94.6-98.2% nucleotide identity and 94.8-96.8% amino acid identity and the carnation isolate (CarMV-C) shared 95.6-97.9% nucleotide identity and 98.0-99.1% amino acid identity. 96.4% nucleotide identity and 96.0% amino acid identity were found between carnation and calla lily isolates from Taiwan. The phylogenetic analysis of the coat protein amino acid sequences indicated that Zan-12B belongs to genotype II while CarMV-C was grouped into genotype III. Base on the results of the coat protein sequence analysis along with the serological assays and electron microscopic examination indicated that the virus Zan-12B isolated from calla lily is an isolate of CarMV and this is the fist report of CarMV infection in calla lilies. In addition, our results also demonstrate that the virus infecting carnation is an isolate of CarMV and this is the first report of CarMV infecting carnation in Taiwan.
| Identifer | oai:union.ndltd.org:TW/092NCHU0363017 |
| Date | January 2004 |
| Creators | Ching-yi Lin, 林靜宜 |
| Contributors | Fuh-Jyh Jan, 詹富智 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 36 |
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