碩士 / 國立彰化師範大學 / 生物學系 / 92 / Thallus of Grateloupia filicina was used for investigating the correlation of polyamines and metabolism of floridean starch under a photoperiod, a long-term darkness and two salt stress(15 ‰, 45 ‰). In photoperiod experiment which was under intermittent illumination of 12 hr light and 12 hr darkness, floridean starch level was found unchanged at the beginning of the light period. Soluble sugar, putrescine (Put) content and activity of two floridean starch degrading enzyme (α-glucosidase (EC 3.2.1.20) and phosphorylase (EC 2.4.1.1)) evidently decreased simultaneous, and protein content obviously increased. During the following dark period, there was significantly declined in floridean starch and protein content, and slightly decreased in soluble sugar. On the contrary, Put content and activity of two floridean starch degrading enzyme remarkably increased, and Put level was accumulated to a maximum value(123.76±4.46 nmol/Fw g) at the 20th hr of photoperiod.The experiment of long-term darkness indicated floridean starch and protein content, phosphorylase activity showed a declined pattern, soluble sugar decreased at the 4th day, Put content and α-glucosidase activity slightly raised during the first 3 days of darkness, and then rapidly declined.
In the experiment of salt stress used 15 ‰ seawater as hyposaline stress, 45 ‰ seawater as hypersaline stress and 30 ‰ seawater as control. Thallus was treated with Put and it’s synthetic inhibitors (D-arginine (D-Arg) and methylornithine(MO)) for 24 hr, then removed to cultural medium without Put and it’s synthetic inhibitors. The results indicated growth rate reduced, soluble sugar and protein increased at 45 ‰ salinity. The change of specific growth rate and ingredients at 15 ‰ were similar to control. Put synthetic inhibitor, D-Arg, could significantly decrease Put content and specific growth rate. There wae no effect in MO. At 45 ‰ salinity, the specific growth rate of thallus treated with Put was highter than at 30 ‰ salinity. In addition, activity of floridean starch degrading enzyme and soluble sugar were inhibited by D-Arg, and increaseing proline level at 45 ‰. Due to 24 hr short-term significant treatment effect may decline with time,and an additional Put and it’s synthetic inhibitor were added to the medium of 30 ‰ and 45 ‰ salinity, and immediately, analysis it’s physiological change after 24 hr. The results indicated phosphorylase activity showed no significantly different, and α-glucosidase activity, Put, floridean starch, soluble sugar and protein content were more significant than 24 hr short-term treatments.
As a whole, after photoperiod and long-term darkness treatments, change in Put content was consistent with the activity of floridean starch degrading enzyme. In the experiment by adding Put and synthetic inhibitors under salt stress were proved that Put may regulate activity of floridean starch degrading enzyme, and to stimulate the floridean starch degradation.
Key words:
Grateloupia filicina、salt stress、floridean starch、floridean starch degrading enzyme、proline、polyamine、polyamine synthetic inhibitors
| Identifer | oai:union.ndltd.org:TW/092NCUE5112021 |
| Date | January 2004 |
| Creators | 許哲溢 |
| Contributors | , 林忠毅, 王瑋龍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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