碩士 / 國防醫學院 / 生理學研究所 / 92 / Abstract
C-phycocyanin (CPC), a major component of Spirulina Platensis, is widely used in many nutrition dietary supplements. Previous study have indicated that CPC prossesses anti-inflammatory effect. However, the true anti-inflammatory mechanism of CPC is still unclear. Therefore, the first aim of this study was to evaluate whether CPC has anti-inflammatory and analgesic effect in a rat model of carrageenan-evoked thermal hyperalgesia, and further investigated the possible mechanisms involved. Our results showed that pre- or post-treatment with CPC significantly inhibited the carrageenan-evoked thermal hyperalgesia. The possible mechanisms may be associated with the decreased formation of nitric oxide and prostaglandin E2 due to attenuation of elevated inducible nitric oxide synthase (iNOS) and cyclooxgenase-2 (COX-2) protein expression in carrageenan-injected rat paws. Furthermore, the suppression of tumour necrosis factor-and myeloperoxidase activity in carrageenen- injected paws may be also involved in its beneficial effect.
Although CPC has been reported to prevent cardiovascular diseases, the effect of CPC on platelet aggregation is still unreported. Thus, the second aim of this study was to investigate whether CPC has antiplatelet effect and further explore the possible mechanisms involved. Preincubation of rabbit washed platelet with CPC (1-50g/ml) dose-dependently inhibited the platelet aggregation induced by arachidonic acid (AA, 100M) or collagen (4g/ml) with IC50 about 10g/ml. Our data further indicated that anti-aggregatory mechanisms of CPC may include (1) The inhibition of thromboxane B2 formation induced by AA or collagen may be through suppressing cycloxygenase and thromboxane synthase activity of platelets (2) The increased formation of cyclic AMP may be due to inhibition of cyclic AMP phosphodiesterase activity (3) increase of platelet membrane fluidity (4) attenuation of the platelet surface GP IIb/IIIa expression (5) suppression of intraplatelet calcium mobilization.
Hopefully, this study can provide further valuable information of CPC for treating these inflammatory, hyperalgesic or hyperaggregatory —related diseases.
| Identifer | oai:union.ndltd.org:TW/092NDMC0116002 |
| Date | January 2004 |
| Creators | Yu-Ling Kuo, 郭昱玲 |
| Contributors | 周志中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
Page generated in 0.0063 seconds