碩士 / 國立屏東科技大學 / 水產養殖系 / 92 / In recent years, the infection disease caused by neuron necrosis virus (NNV) and grouper iridovirus (GIV) dozen of important aquaculture industries in the world. The species affected include fish, amphibian and reptile, and the habitat covered from temperate zone to tropical zone and from seawater to freshwater. To prevent and treat this virus infections are therefore top urgent issues right now. In this study, we prepared formalin inactivated vaccines for yellow grouper neuron necrosis virus (YGNNV) and grouper iridovirus (GIV). The result showed, after injection in combinations of various vaccines treatments injected GIV, YGNNV, and YGNNV+GIV vaccines, respectively the specific immunoreaction of treated seaperch’s tissue fluid were detected by the enzyme-linked immunosorbent assay (ELISA). Soaking in inoculated vaccine three times, the tested fry exhibited non-specific immune response. While soaking in same vaccine twice and injecting it one, the fry showed obvious specific immune response (p<0.05). After three months of inoculated vaccine, the fry injected with YGNNV+GIV treatment has the best survival rate during YGNNV and GIV virus challenge test. This fact implies that mixing vaccines (YGNNV+GIV) possess not only the specific immunoreaction but non-specific protective effects. For the accumulative mortality in field trials, we found that inoculated vaccine injection has a better protective and higher safety effects than soaking one.
| Identifer | oai:union.ndltd.org:TW/092NPUST086010 |
| Date | January 2004 |
| Creators | Ching-Yi Tsai, 蔡靜宜 |
| Contributors | Chi-Yao Chang, Shinn-Pyng Yeh, 張繼堯, 葉信平 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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