碩士 / 國立清華大學 / 分子與細胞生物研究所 / 92 / Abstract
The A-to-I (adenosine to inosine) RNA editing catalyzing deamination of adenosines to inosines on pre-mRNA is a post-transcriptional modification mechanism which increases complexities of a gene product. Vertebrates express three kinds of ADARs (adenosine deaminase that act on RNA), ADAR1, ADAR2, and ADAR3. ADAR2 knock-down zebrafish embryos developed abnormally. The abnormal phenotypes include small eyes, yolk sac defects, touch insensitivity, locomotive defects, growth retardation, and death before 7 dpf. The temporal and spatial expression patterns of HuC and the sodium channel, zNav1.6, of wildtype and morphant zebrafish embryos were compared to delineate a possible involvement of ADAR2 in the neurogenesis. The numbers and distribution patterns of primary neurons in the morphant differed from those of the wildtype embryos. Comparisons of crystallin αA expression patterns between wildtype and morphant showed that the lens development might be retarded. TUNEL assay and acridine orange staining were performed to study the patterns of apoptosis in the embryos. Excessive and widespread apoptosis were detected in the morphant embryos between 20 to 36hpf. Quantitative RT-PCR analysis revealed that the expression level of pro-apoptotic zBax mRNA increased significantly in the 16-36 hpf morphants, whereas the mRNA levels of zBad, zBcl-xl and zMcl-1a remained the same between wildtype and morphant. These results suggest ADAR2 plays an essential role in the embryogenesis and is required for the developments of neuron, lens, and the regulation of apoptosis.
| Identifer | oai:union.ndltd.org:TW/092NTHU5061010 |
| Date | January 2004 |
| Creators | Chiung -Wen Ou, 歐瓊雯 |
| Contributors | Wei-Yuan Chow, 周姽嫄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 46 |
Page generated in 0.0113 seconds