碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Abstract
This study examined the immune response of the white shrimp Litopenaeus vannamei (8.4～12.4 g) and its resistance against Vibrio alginolyticus when the shrimp were injected with hot-water extract of Gracilaria tenuistipitata or Gelidium amansii at a dose of 4 or 6 μg g-1. The immune parameters included total haemocyte count (THC), phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase activity, phagocytic activity and clearance efficiency.
L. vannamei injected with hot-water extract of G. tenuistipitata at 6 μg g-1 increased its THC、phenoloxidase activity and respiratory burst after 1 and 2 days. L. vannamei injected with hot-water extract of G. tenuistipitata at 6 μg g-1 increased its superoxide dismutase activity after 1 day. L. vannamei injected with hot-water extract of G. tenuistipitata at 6 μg g-1 increased its phagocytic activity and clearance efficiency to V. alginolyticus after 1 day. In another experiment, L.vannamei which had been injected with hot-water extract of G. tenuistipitata, were challenged with V. alginolyticus at 2×106 colony-forming units (cfu) shrimp-1 and then placed in seawater of 34 ‰. The survival of shrimp that received hot-water extract of G. tenuistipitata at 6 μg g-1 was significantly higher than that of shrimp injected saline and the control shrimp at the termination of the experiment (6 days after the challenge). It is concluded that hot-water extract of G. tenuistipitata at a dose of 6 μg g-1 causes an increases in the immune ability of L. vannamei and resistance from V. alginolyticus infection.
L. vannamei injected with hot-water extract of G. amansii at 6 μg g-1 increased its THC、phenoloxidase activity and respiratory burst after 1 day. No significant difference in superoxide dismutase activity was observed among the shrimp injected with saline and those injected with hot-water extract of G. amansii at doses of 4 and 6 μg g-1. L. vannamei injected with hot-water extract of G. amansii at 6 μg g-1 increased its phagocytic activity and clearance efficiency against V. alginolyticus after 2 days. In another experiment, L. vannamei which had been injected with hot-water extract of G. amansii were challenged with V. alginolyticus at 2×106 cfu shrimp-1, and then placed in seawater of 34 ‰. The survival of shrimp that received hot-water extract of G. amansii at 6 μg g-1 was significantly higher than that of shrimp injected saline and the control shrimp after 6 days.
The present study indicated that both hot-water extracts of G. tenuistipitata and G. amansii can enhance the immune ability of L. vannamei. However a continuous dose is necessary to maintain the immune ability of shrimp. Further research is needed to evaluate the immunostimulatory effects of L. vannamei and its resistance against V. alginolyticus when shrimp fed a diet containing either hot-water extract of G. tenuistipitata or G. amansii .
Key worlds: Litopenaeus vannamei, Gracilaria tenuistipitata, Gelidium amansii, hot-water extract, Vibrio alginolyticus, immune parameters, challenge, phagocytic activity, clearance efficiency
| Identifer | oai:union.ndltd.org:TW/092NTOU5086007 |
| Date | January 2004 |
| Creators | Wen-Ying Hou, 侯彣縈 |
| Contributors | Jiann-Chu Chen, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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