碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Abstract
The objective of this research is aimed to document the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under the changes in salinity and temperature.
White shrimp L. vannamei held in 25 ‰ were injected with TSB-grown V. alginolyticus（1 x 104 cfu/shrimp), and then transferred to 5, 15, 25 and 35 ‰. Over 48-96 h, the mortality of V. alginolyticus-injected shrimp held in 5 ‰ and 15 ‰ was significantly higher than that of shrimp held in 25 ‰ and 35 ‰, and the mortality of V. alginolyticus-injected shrimp held in 5 ‰ was the highest. Shrimp held in 25 ‰ and then transferred to 5, 15, 25（control) and 35 ‰ were examined for THC（total haemocyte count), phenoloxidase activity, respiratory burst, superoxide dismutase（SOD) activity, phagocytic activity and clearance efficiency to V. alginolyticus after 12-72 h.The THC, phenoloxidase activity, respiratory burst, SOD activity, phagocytic activity and clearance efficiency decreased significantly for the shrimp held in 5 and 15 ‰ after 12 h. It is concluded that the shrimp transferred from 25 ‰ to low salinity（5 and 15 ‰) had reduced immune ability and decreased resistance against V. alginolyticus infection.
White shrimp L. vannamei held in 28 °C at 25 ‰ were injected with TSB-grown V. alginolyticus（1 x 105 cfu/shrimp), and then transferred to 20, 24, 28, 32 °C. Over 144 h, the mortality of V. alginolyticus-injected shrimp transferred to 32 °C was significantly higher than that of shrimp transferred to 20, 24, 28 °C（control). Shrimp held in 25 ‰ and 28 °C, then transferred to 20, 24, 28 and 32 °C were examined for THC（total haemocyte count), phenoloxidase activity, respiratory burst, superoxide dismutase（SOD) activity after 12-96 h. Both THC and phenoloxidase activity increased after 12 h, but decreased after 24 h for the shrimp transferred to 32 °C. Both respiratory burst and SOD activity decreased for the shrimp transferred to 20 °C after 12-48 h. It is concluded that the shrimp transferred from 28 °C to higher temperature（32 °C) had reduced immune ability and decreased resistance against V. alginolyticus infection.
Therefore, changes in salinity（from 25 ‰ to 5 and 15 ‰) and temperature（from 28 °C to 32 °C) cause reduced immune ability of white shrimp L. vannamei and lead to decreased resistance against V. alginolyticus.
Keywords: Litopenaeus vannamei, Vibrio alginolyticus, Salinity, Temperature, Susceptibility, total haemocyte count, Phenoloxidase activity, Respiratory burst, Superoxide dismutase activity, Phagocytic activity, Clearance efficiency
| Identifer | oai:union.ndltd.org:TW/092NTOU5086008 |
| Date | January 2004 |
| Creators | Long-Uong Wang, 王融雍 |
| Contributors | Jiann-Chu Chen, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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