碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Abstract
This study examined the immune response of the white shrimp Litopenaeus vannamei, and its resistance against Vibrio alginolyticus when the shrimp were injected with chitin and chitosan.
L. vannamei injected with chitin at dose of 4 μg g-1 or 6 μg g-1 were examined for the immune parameters after 1, 2, 4 and 6 days. L. vannamei injected with chitin at a dose of 6 μl g-1 increased significantly its total haemocyte count (THC) after 2 days. L. vannamei injected with chitin at a dose of 6 μg g-1 increased significantly its phenoloxidase activity and respiratory burst after 1 day. L. vannamei injected with chitin at 4 μg g-1and 6 μg g-1 increased significantly its phagocytic activity and clearance efficiency against V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with chitin, were challenged with V. alginolyticus at 2×106 colony-forming units (CFU) shrimp-1 and then placed in seawater of 34 ‰, the survival rate of shrimp that received chitin at dose of 4 μg g-1 and 6 μg g-1 after 1 day was significantly higher as compared to the shrimp that received saline and the control shrimp. It is therefore concluded that L. vannamei that received chitin at a dose of 4 μg g-1 increased its immune ability and resistance against V. alginolyticus infection.
L. vannamei injected with chitosan at dose of 2 μg g-1 or 4 μg g-1 were examined for the immune parameters after 1, 2, 4 and 6 days. L. vannamei injected with chitosan at 2 μg g-1 and 4 μg g-1 increased its total haemocyte count significantly as compared to the shrimp received saline and the control shrimp after 1 day. L. vannamei which was injected with chitosan at 2 μg g-1 and 4 μg g-1 increased significantly its phenoloxidase activity and respiratory burst after 1 day. L. vannamei injected chitosan at a dose of 4 μg g-1 significantly increased its phagocytic activity and clearance efficiency to V. alginolyticus after 2 days. In another experiment, L. vannamei which had been injected with chitosan, were challenged with V. alginolyticus at 2×106 CFU shrimp-1 and then placed in seawater of 34 ‰. The survival rate of shrimp that received chitosan at 2 μg g-1 and 4 μg g-1 after 2 days increased significantly as compared to the shrimp that received saline and the control shrimp. It is therefore concluded that L. vannamei that received chitosan at a dose of 2 μg g-1 increased its immune ability and resistance against V. alginolyticus infection.
The present study indicated that both chitin and chitosan can enchance the immune ability of L. vannamei. A continuous dose is necessary to maintain the immune ability of L. vannamei. Further research is needed to evaluate the immune ability of L. vannamei and its resistance against V. alginolyticus when shrimp fed a diet containing chitin or chitosan.
Key words: Litopenaeus vannamei, chitin, chitosan, Vibrio alginolyticus, immune parameters, challenge, phagocytic activity, clearance efficiency.
| Identifer | oai:union.ndltd.org:TW/092NTOU5086011 |
| Date | January 2004 |
| Creators | Shi-Hong Wang, 王世弘 |
| Contributors | Jiann-Chu Chen, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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