碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 92 / Methyl farnesoate (MF), an analogue of the insect juvenile hormone III, has been implicated to play a vital role in the regulation of the growth and reproductive development in crustaceans. Farnesoic acid O-methyl transferase (FAMeT) is the key enzyme involved in catalyzing the final step in the MF biosynthetic pathway. In this studies, we report the cloning and characterization of the cDNA encoding the deduced FAMeT of the tiger shrimp (P. monodon) and white shrimp (L. vannamei). The degenerated primers were designed from FAMeT gene in sand shrimp (M. ensis) and other crustaceans. The reverse-transcriptase polymerase chain reaction (RT- PCR) and 5’、3’-RACE was performed cloning eyestalk of FAMeT cDNA were deduced 280 amino acid and molecular weight is 32 kDa. The shrimp FAMeT mRNA is widely distributed in many tissues with the highest expression in the testis and ovary. In molting cycle, the highest level of FAMeT mRNA is found in the intermolt stage (C stage) and dropped steadily during premolt stage (D0、D1 stage)。After eyestalk ablation, the level of FAMeT mRNA expression increased gradually follow by time increase, it suggests that FAMeT may play an important role in the regulation of eyestalk neuropeptides in penaeid shrimp reproduction system. Production of recombination FAMeT protein in E. coli expression system is about 35 kDa. In present study, it is possible to use high efficient E.coli expression system to produce low cost FAMeT recombinant protein.
| Identifer | oai:union.ndltd.org:TW/092NTOU5086029 |
| Date | January 2004 |
| Creators | Yi-Yu Lee, 李宜諭 |
| Contributors | Jenn-Kan Lu, 陸振岡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 101 |
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