碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 92 / ARNT is a member of bHLH/PAS family, which dimerizes with other members of bHLH/PAS family proteins, including HIF, SIM, CLOCK, AHR, to form heterodimers and regulate the transcriptional activities of target genes. To investigate the functions of ARNT in vertebrate development, the arnt2a/b/c-specific morpholino oligonucleotide was injected into fertilized embryo at 1-cell stage to block translation of ARNT2A/B/C and observe the effects on tissue development. Via whole mount in situ hybridization, tissue section and scanning electron microscope, it appeared that the development of neural retina and lens epithelium was disrupted in arnt2a/b/c-knockdown embryo. A large number of primordial retina cells were retained in undifferentiated state. In addition, neuroD, one of the key regulator of neuronal differentiation, did not express in the retinal region of arnt2a/b/c morphant. The optic nerve-specific gene exhibited abnormal expression pattern which cause optic nerve projection with wrong destinations. Accodingly, it appeared that arnt2a/b/c played essential roles in visual neuronal cell differentiation.
| Identifer | oai:union.ndltd.org:TW/092NTOU5111005 |
| Date | January 2004 |
| Creators | Yi-Chung chen, 陳宜群 |
| Contributors | Chin-Hwa Hu, 胡清華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 86 |
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