碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 92 / Recently, a novel bHLH-PAS transcriptional factor family was revealed to play an important role in vertebrate development. SIM, HIF and AHR can form heterodimers with a central bHLH/PAS partner protein, ARNT, to modulate variety of biological functions, including neurogenesis, angiogenesis and vasculogenesis, and biological circadian rhythm.
Previous studies in our laboratory have shown that repression of arnt2a/b/c or hif1a+hif3a by morpholino oligonucleotides (MO) caused serious defect in embryonic cranial phenotype. In this study, I observed the cranial defect of arnt2a/b/c-knockdown morphant embryos by scanning electric microscope (SEM) and found significant defect in the olfactory pit comparing to the wild type embryos. Hence, we proposed that those genes may involved in olfactory organ development.
In order to understand the detail regulatory mechanism, we have investigated the expression patterns of several olfactory relative marker genes, including omp, anxV, coe2, neuroD, emx1 and dlx3, by in situ hybridization. It appeared that the development of olfactory organs have been affected significantly in the early stages of the arnt2a/b/c morphant embryos. The repression of neural crest marker, foxd3, suggested that the neural crest cells are decreased in arnt2a/b/c morphant embryos. It was known that the cranial neural crest cells play important role in inducing olfactory placode development and a portion of them form the olfactory cartilage. This study revealed that arnt2a/b/c are very important in olfactory development. In contrast, there was no significant defect in hif1a+hif3a knockdown morphant embryos. It suggested that the hif1a+hif3a antisense morpholino affected the olfactory development indirectly.
In addition to the olfactory-related markers, the expression patterns of a number of neural maker genes, such as ngn1, coe2, l1.1, were also affected in the arnt2a/b/c morphant embryos. It suggested that ARNT2A/B/C play essential roles in neural system development.
| Identifer | oai:union.ndltd.org:TW/092NTOU5111006 |
| Date | January 2004 |
| Creators | Jian-Shiung Wu, 吳建勳 |
| Contributors | Chin-Hwa Hu, 胡清華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 86 |
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