碩士 / 國立臺灣海洋大學 / 食品科學系 / 92 / Abstract
To investigate the utilization of β-carotene and its bioconversion to vitamin A in juvenile grass shrimp (Penaeus monodon), two growth trials were conducted. In experiment I, semi-purified diet contained seven levels of β-carotene (βC) (0, 30,000, 60,000, 90,000, 120,000, 150,000, 200,000 IU/kg diet) together with a reference diet containing 8,400 IU/kg diet of vitamin A (retinyl acetate, VA) were each fed to triplicate groups of shrimp (mean initial weight: 0.088 ± 0.002 g) for 8 weeks. Weight gain (WG) was significantly (P < 0.05) higher in shrimp fed diet with 150,000 IU βC/kg than shrimp fed diets with 30,000, 200,000 IU βC/kg and the unsupplemented control diet. Body vitamin A concentration was higher in shrimp fed diets with 60,000 and 90,000 IU βC/kg than shrimp fed diets with≧ 120,000 IU βC/kg and≦ 30,000 βC/kg. Body β-carotene concentration was higher in shrimp fed diets with≧ 150,000 IU βC/kg than the other dietary groups. Body astaxanthin concentration was highest in shrimp fed diets with 30,000 and 60,000 IU βC/kg, followed by shrimp fed diets with 90,000, 120,000 IU βC/kg and the control diet, and lowest in shrimp fed diets with≧ 150,000 IU βC/kg and the reference diet. The hepatopancreatic 15,15’-oxygenase activity (βCOase) was higher in shrimp fed diet supplemented with 120,000 IU βC/kg than shrimp fed the control and the reference diets. Analyses by polynomial regression of weight gain and oxygenase activity of the shrimp indicated that the maximum responses occurred at the 146,500 and 138,300 IU βC/kg, respectively suggesting that the bioconversion rate (mass basic) of β-carotene to vitamin A in grass shrimp is about 1/34-1/36. The bioconversion rate estimated by the body vitamin A, β-carotene and astaxanthin concentration are 1/5 - 1/10 and 1/32 - 1/35 as the β-carotene supplementation levels at 30,000 - 90,000 and 120,000 - 150,000 IU/kg, respectively. In experiment II, semi-purified diet supplemented with three levels of either β-carotene (30,000, 60,000, 90,000 IU/kg diet) and vitamin A (5,000, 10,000, 15,000 IU/kg diet) were each fed to triplicate groups of shrimp (mean initial weight: 0.270 ± 0.012 g) for 8 weeks. WG was significantly higher in shrimp fed diet with 10,000 IU VA/kg than shrimp fed diet with 30,000 IU βC/kg. The 15,15’-oxygenase activity was highest in shrimp fed diet with 5,000 IU VA/kg, followed by shrimp fed diets with 10,000 IU VA/kg and≧ 60,000 IU βC/kg, and lowest in shrimp fed diet with 30,000 IU βC/kg. Body astaxanthin concentration was higher in shrimp fed diet with 60,000 IU βC/kg than shrimp fed diet with 10,000 IU VA/kg. The body vitamin A concentration of the β-carotene and vitamin A fed groups were generally increased as the supplementation level increased. The slope of the linear regression lines of the β-carotene and vitamin A groups were 0.13 and 1.31, respectively suggesting that the bioconversion rate of β-carotene to vitamin A in grass shrimp is about 1/10.
| Identifer | oai:union.ndltd.org:TW/092NTOU5253015 |
| Date | January 2004 |
| Creators | Chin-Ying Wang, 王姿穎 |
| Contributors | Shi-Yen Shiau, 蕭錫延 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 84 |
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