碩士 / 國立臺灣大學 / 動物學研究所 / 92 / SSN-1 cell line was applied in many research works of nervous necrosis virus (NNV), but it was SnRV-persistently infected. Lee et al. (2002) developed another SnRV-persistently infected cell line SGF-1 by inoculation of the culture supernatant of SSN-1 cells into GF-1 cells. The goal of this study is to observe the interaction of SnRV and NNV during co-infection of SGF-1 cells. Both NNV genomic RNAs were reversely transcribed into cDNA with high conservation and a new stage of NNV in the life cycle was created. The sequences which were 40% to 50% identity with the sequence of primer binding site (PBS) of SnRV reverse transcriptase (RT) were found in NNV genomic RNAs. However, the both sequences were in the middle site of NNV genomic RNAs. Therefore, further experiments will be necessary to find the real PBS of SnRV RT. During the co-infection in NNV-infected SGF-1 cells, NNV cDNA was detected since day 3, and the amount of NNV cDNA increased daily. In the other hand, the SnRV RNA was not detected since day 4, and the amount of SnRV RNA decreased day by day. The titers of the supernatants collected from NNV-infected SGF-1 cells in GF-1 cells, were obviously higher than that in SGF-1 cells and determined in day 3 to day 5. It was suggested that the change of the titer might be linked up the exciting of SnRV receptor. Besides, if NNV RNA was reversely transcribed, that depended on what the host was. Finally, the data showed NNV and SnRV could co-infected in vivo when artificial infected. But there was not any co-infection in nature been found.
| Identifer | oai:union.ndltd.org:TW/092NTU00312017 |
| Date | January 2004 |
| Creators | Li-Han Chen, 陳立涵 |
| Contributors | 齊肖琪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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