碩士 / 國立臺灣大學 / 海洋研究所 / 92 / Marine vibrios have the most abundance of the physiological and ecological function which are most widely distributed in marine ecosystems.Thus,it''s very important to study the diversity of marine vibrios for studying the diversity of marine ecosystems.
Attempted to know the diversity of marine vibrios in coastal seawater of northern Taiwan,the seawater samples were collected every month from Fei-Zuei Bay, Badouzih, Shenao and Nan-Ya of Keelung, Taiwan during period of November 2003 to February 2004.
Plate counting values of the marine vibrios in the samples were mostly in the range of 101 to 102 cells/ml.The values usually accounted for 10﹪of the heterotrophic bacteria.628 bacterial strains were isolated from the counting plates in the study.But 433 of these bacterial strains have the ability of fermentation for glucose.They could be divided into twenty-three groups according to the results of the following tests：Gram reaction, requirement of Na＋ for growth, fermentation of inositol, D-Arabinose, dulcitol and Xylose.43 strains of group I and group Ⅱ were Gram-positive.Obviously,they were not marine vibrios.390 of the twenty-one groups all have the character of marine vibrios.
From each of the twenty-one groups,we chose one strain for 16S rDNA sequence and two strains for 16S rDNA PCR-RFLP after the response by three kinds of enzyme（Dde I,Rsa I,Sca I）.After analysising the results,the twenty-one groups could be further divided into seventy species.And only three pairs of group Ⅲ,group Ⅵ and group Ⅷ compelet the same after 16S rDNA PCR-RFLP.Thus,most of the groups could not contain only one specie.The study seems that it´s been exist the high diversity of species of marine vibrios in coastal seawater of northern Taiwan.
| Identifer | oai:union.ndltd.org:TW/092NTU05274014 |
| Date | January 2004 |
| Creators | Yu-Chen Lin, 林育震 |
| Contributors | 謝文陽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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