碩士 / 靜宜大學 / 食品營養研究所 / 92 / Abstract
Astaxanthin have been showed many physiological functions, such as nutrition requirement, reproduction performance, stabilization of protein and enzyme activity, antioxidative ability, and immunity. The purpose of the study was to examine the effects of Astaxanthin on learning memory ability and antioxidative status in different age of mice. Three and Six month-old senescence accelerated mice (SAMP8) were divided into three groups: casein diet group (control group) and casein diet supplemented with 0.01%, 0.025% Astaxanthin (experimental group respectively). After 16 weeks of feeding, body weight, food intake, aging score, open field activity test and active shuttle avoidance test were interval performed during the experiment. The biochemical parameters of serum and the liver superoxide dismutase (SOD) were analyzed after sacrificed. The SOD, malondialdehyde (MDA), total thiol concentrations, pathological examination of brain, motility and concentrations of spermatozoa in male mice were also evaluated. The results showed that there were no significant differences in the body weight, food intake, and locomotion among three groups in both 3 and 6-month-old mice, whereas 3-month-old groups showed higher food efficiency. The aging score of control groups were higher than the experimental groups in both 3 and 6-month-old mice (P<0.05). 3 and 6-month-old mice fed with the 0.025% Astaxanthin had significantly better single-trial passive avoidance results(P<0.05). The experimental groups had better active shuttle avoidance response in both 3 and 6-month-old mice. In 6-month-old female mice, the mice were fed with the 0.025% Astaxanthin group that had significantly lower total cholesterol of serum than the control group (P<0.05). The hepatic SOD activity in the 0.025% Astaxanthin group was higher than the group in 6-month-old male mice (P<0.05)；The SOD activity of brain in the 0.025% Astaxanthin group was higher than that of control group in both 6-month-old mice (P<0.05). The MDA concentrations of brain in the experimental groups were lower than control groups in both 3 and 6-month-old mice (P<0.05). The total thiol concentrations of brain in the experimental group were higher than control groups in 6-month-old mice (P<0.05). The β-amyloid protein deposition of brain in the experimental groups were lower than control groups in both 3 and 6-month-old male mice, whereas 3-month-old female mice fed with the 0.025% Astaxanthin group had significantly lower β-amyloid protein deposition of brain than control group (P<0.05). 3-month-old male mice fed with the 0.025% Astaxanthin had significantly better motility of spermatozoa (P<0.05). The concentrations of spermatozoa were no differences in both 3 and 6-month-old male mice. In summary, we conclude the supply of Astaxanthin may improve aging score, learning and memory ability and reducing the brain pathological changed in SAMP8 mice. It is suggested that may due to promote the antioxidative ability and lowering the oxidative injury after Astaxanthin supplementation.
| Identifer | oai:union.ndltd.org:TW/092PU005255006 |
| Date | January 2003 |
| Creators | Chiao-Hsing Chang, 張巧幸 |
| Contributors | Ming-Fu Wang, 王銘富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 160 |
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