碩士 / 大仁科技大學 / 環境管理研究所 / 92 / Paralytic shellfish poisoning (PSP) associated with food poisoning incidents have caused two human fatalities and many illnesses in Taiwan. The sourse of PSP in Taiwan was thought to be the toxic dinoflagellate Alexandrium minutum. To understand the trace elements in walne medium for cell growth and toxin production of A. minutum, the different concentration of copper and iron ion, and vitamin B group were added into the walne medium of A. minutum. It was found that the optimal trace elements for those of A. minutum were as follows : copper ion (Cu2+) 5.0 ppb, ferric ion (Fe3+) 270 ppb, and vitamin B1 200 ppb and vitamin B12 10 ppb. The toxic components of A. minutum were assayed by high performance liquid chromatography (HPLC), and found to contain gonyautoxin (GTX)1-4 only. Among these components, GTX1 and GTX4 were predominate components.
On the other hand, chemicals were commonly used in aquaculture ponds to controlling toxic dinoflagellate. The effects of algicides 【(NH4)2SO4、CuSO4、KMnO4、benzalkonium chloride（BKC）、methylene blue and malachite green】, chitosan and ozone on the growth and toxicity of A. minutum were elucidated. Among the tested chemicals, O3 was found to be the more effective, followed by BKC and (NH4)2SO4. At the effective concentration (EC50) of 24 hrs for A. minutum were as follows : ozone 0.5 ppm, BKC 1.0 ppm, KMnO4 7.5 ppm , malachite green 10 ppm and (NH4)2SO4 11.1 ppm. At the effective concentration (EC50) of 96 hrs for A. minutum were as follows: ozone 0.5 ppm, BKC 1.0 ppm, (NH4)2SO4 1.3 ppm, KMnO4 5.2 ppm, malachite green 10 ppm, Methylene blue 35 ppm, CuSO4 100 ppm, D(+)glucosamine (pH 6.0) 200 ppm, and chitosan (pH 6.0) 1000 ppm. The total toxicity of A. minutum in each treatment were lower than control treatment, whereas the cell toxicity in low concentration of chemical treatments (except Glucosamine and Chitosan) was higher than control treatment. The toxic components of A. minutum for each treatment were assay by HPLC, and found to contain GTX1-4 only. Among these toxin components, GTX1 and GTX4 were the predominate components.
| Identifer | oai:union.ndltd.org:TW/092TAJ05700008 |
| Date | January 2004 |
| Creators | Chiu-Chu Hwang, 黃秋菊 |
| Contributors | Yung-Hsien Tsai, 蔡永祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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