碩士 / 東海大學 / 食品科學系 / 92 / Trehalose is found in diverse organisms . It is commonly considered a storage compound but more recently has been recognized to function mainly as a protectant for maintaining vital structures in the cytosol under stressful conditions such as extreme temperatures, drought, and desiccation .It might do so by stabilizing membranes and protecting enzymes. Trehalose is also expected to be used for various applications in the fields of food industry ,cosmetics and pharmaceuticals. Trehalase catalyzes the hydrolysis of trehalose into glucose. It plays important role in regulating trehalose content in vivo and can be used for the analysis of trehalose in vitro.
In this study , a trehalase gene (Os-TRE) protein was isolated from rice encoding a 563-amino-acid. The predicted molecular weight was 62 kDa and its pI value was 5.49. Os-TRE gene was overexpressed in Escherichia coli system and the recombinant protein was shown to possess trehalase activity. This is the first plant trehalase gene which was successfully expressed as an active recombinant protein. In the future, a antisense RNA technology or RNA method can be used to suppress trehalase activity, which might increase the trehalose content in rice plants and confer tolerance to different abiotic stresses .
| Identifer | oai:union.ndltd.org:TW/092THU00253011 |
| Date | January 2004 |
| Creators | Meng-Jen Lin, 林孟甄 |
| Contributors | Ken-Yuon Li, Jei-Fu Shaw, 李根永, 蕭介夫 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 99 |
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