碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 93 / White Spot Syndrome Virus (WSSV) is a large, circular dsDNA virus , which may infect shrimps and other crustaceans. This virus is a member of the genus Whispovirus within a new virus family called Nimaviridae, referred to the thread-like polar extension on the virus particle. The complete sequences of the WSSV genomes have now been published as three different isolates. However, most of WSSV open reading frames , which can encode proteins, have no homology to any known proteins or motifs. With in the genome of the Taiwan WSSV isolate, RT-PCR and microarry analyses showed that these ORFs (WSSV071, WSSV302,WSSV338) were putative as very late genes. Therefore, in this study, we try to construct the recombinant plasmids containing the ORFs, and to express them by transformation into Escherichia coli, BL-21(DE3). Until now, the His-tagged fusion proteins containing the WSSV071, WSSV302 and WSSV338 respectively were expressed successfully and purified to produce antiserums for Western blot analysis. In addition, we construct the recombinant plasmids containing the ORFs, and to express them by transfection into Spodoptera frugiperda (Sf9) insect cells .The efficiency of transfection were detected with EGFP expression using the fluorescent microscopy.
| Identifer | oai:union.ndltd.org:TW/093CNUP5111014 |
| Date | January 2005 |
| Creators | Ming-Hung Huang, 黃明宏 |
| Contributors | Nai-Yueh Tien, 田 乃 月 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
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