碩士 / 嘉南藥理科技大學 / 生物科技研究所 / 93 / The aim of the present study was to evaluate the influence of ethanolic extracts of Noni leaves on the antioxidative properties of the blood and liver of rats exposed to cyclophosphamide. Eighty adult male Sprague-Dawley rats were divided into eight groups randomly and fed with AIN-76 diet for 30 days. One group was injected intraperitoneally with phospahte saline buffer on the fifteenth day and served as the control group（C）. The LE1 group was indicated that the rats received 95% ethanolic extracts of Noni’s leaves（LE95） at a dose of 0.2g/kg body weight per day. The others were fed with 50% ethanolic extracts of Noni’s leaves at a dose of 0.2（CPLE50）, LE95 at a dose of 0 （CP）, 0.2 （CPLE1）, 0.4（CPLE2）, 1（CPLE5）g/kg body weight or fed with silymarin（CPSL）at a dose of 0.2 g/kg body weight per day, and injected intraperitoneally with cyclophosphamide in phospahte saline buffer on the fifteenth day. After thirty days of administration, rats were sacrificed by ether anaesthesia. Blood of rat’s abdominal artery were drawn with an anticoagulant. Red blood cells, plasma and liver tissues were used for antioxidant enzymes or non-enzymatic antioxidants assays. Further study was to prove the 95% ethanolic extracts of Noni leaves（LE95）in the protection ability of lipid peroxidation and oxidative damage of protein caused by cyclophosphamide.
The first statistical analysis was to evaluate the antioxidative potential of the ethanolic extracts of Noni leaves（LE95 and LE50）in comparison with silymarin. The data were analyzed by one-way ANOVA with Duncan’s test for multiple comparisons to determine significance among C, CP, CPLE1, CPLE50, CPSL groups. The results implied that ethanolic extracts of Noni leaves had better antioxidative properties against oxidative stress induced by cyclophosphamide than silymarin which is well known as hepatic protective drug. Furthermore, LE95 appeared the marked and consistent antioxidative protection effects in rat livers and blood system, but not in LE50.
To further examine whether antioxidative protection effects of LE95 were also affected by the doses of LE95 and the interaction of LE95 with cyclophosphamide, a nested experimental design was evaluated. The data were analyzed by one-way and multiway ANOVA among C, CP, LE1, CPLE1, CPLE2, CPLE5 groups. The results indicated as following.（1）The rats received LE95 at a dose of 0.2g/kg body weight per day had higher the glutathione peroxidase activity（GPx） during 7 days and higher the glutathione reductase activity（GRd）during 15 days after cyclophosphamide administration in red blood cells than the other groups. In addition, a decrease of aspartate aminotransferase activity（AST）, normal hepatic tissue biopsy and normal relative liver weight were observed in rats dosed with 0.2g/kg body weight of LE95.（2）The rats received LE95 at a dose of 0.4g/kg body weight per day had higher the GPx during 7 days and higher the catalase（CAT）and superoxide dismutase activity（SOD）during 15 days after cyclophosphamide administration in red blood cells. In addition, a decrease of AST, increase hepatic NPSH, TSH contents and increase scavenging ability of DPPH in rat liver were observed. However, the dramatic increase of relative spleen weight in CPLE2 group. The result indicated that the rats received LE95 at a dose of 0.4g/kg body weight may act against hemolytic anemia by enhancement of the splenocyte proliferation rate.（3）The rats received LE95 at a dose of 1g/kg body weight per day could decrease AST, increase hepatic NPSH, TSH contents and increase scavenging ability of DPPH in rat liver. However, higher MCH value, higher carbonyl group’s content of plasma, and lower CAT during 15 days after cyclophosphamide administration in red blood cells were observed. These results implied that higher dosage of LE95 may appear aggravating oxidative damage in blood system of rats.
In conclusion, our results suggest that LE95 had some protection effects on blood and liver in rats from oxidative stress. However, the effectiveness of LE95 relates to the dose of usage.
| Identifer | oai:union.ndltd.org:TW/093CNUP5111022 |
| Date | January 2005 |
| Creators | Chih-Hsien Chen, 陳智賢 |
| Contributors | Dong-Bor Yeh, Shih-Ying Chen, 葉東柏, 陳師瑩 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 111 |
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