碩士 / 國立中興大學 / 生物產業機電工程學系 / 93 / For the monitoring of culture ponds, only the data of water quality such as water temperature, pH value, salinity, and the concentrations of nitrogen compounds are not enough to represent the complex environment of culture pond while the information of microbial community are needed. The culture-dependent methods of studying microorganisms are time consuming and usually get a bias result. Molecular technologies like FISH, PCR-DGGE were used to analyze the microbial community in water and sediment of the culture pond. The results indicated that the variations of microbial community were not obvious while the water quality of the culture pond were stable during the period of this study. The water exchange between day 27 and day35 caused the decrease in total counts of bacteria and the proportion of α, β, and γ subdivision of Eubacteria. The probe designed for the ammonia-oxidizing bacteria in β- Proteobacterial hybridize 8.4% to 15.39% of total bacteria, that showed AOBs were not the dominant of fish farm. The reason might be the low concentration of ammonia. The amount of Annamox were higher in the sediment than in the water but still less than 1.5%. Specific DNA fragments of microorganisms in the water and sediment were amplified from extracted DNA by PCR and then analysed by DGGE. The result showed the variation of microbial community in the water and the microbial community were different between the water and the sediment.
| Identifer | oai:union.ndltd.org:TW/093NCHU0415031 |
| Date | January 2005 |
| Creators | Lin-Li Wang, 王林立 |
| Contributors | Jiunn-Ming Chen, Chun-Hsiung Hung, 陳俊明, 洪俊雄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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