碩士 / 國立成功大學 / 細胞生物及解剖學研究所 / 93 / Synthetic glucocorticoid-dexamethasone, have been widely used to accelerate pulmonary maturation and inhibit lung inflammation of preterm infants. However, follow-up studies showed that preterm infants received steroid therapy result in neurologic dysfunction, less cortical gray tissue and total brain volume. In our pilot studies, dexamethasone injected postnatal day one rat pups either with high (0.5 mg/kg) or low dose (0.2 mg/kg) result in the cellular irregularity, dendritic atrophy, increased number of pyknotic cells and vascularity in the hippocampus. The pathological changes can be observed predominantly in the CA1 subregion and the granular cell layer of dentae gyrus. Acupuncture has been applied in clinical treatment of various diseases in Oriental medicine. Growing evidence shows acupuncture enhance cell proliferation in dentate gyrus either in the global ischemic gerbil, in maternally separates stressed rats, or in the diabetic rat. Zusanli (ST-36), an acupoint located 3 mm lateral and distal to the anterior tubercle of the tibia, has been examined in various studies and has been proved to have various effects, including efficacy in the treatment of cerebrovascular diseases in humans and animals. Therefore, purpose of the study is to evaluate the effect of acupuncture at ST-36 enhanced the cell proliferation of dexamethasone damaged neonatal hippocampal formation. Through the 5-bromo-2’-deoxyuridine (BrdU) immunocytochemic stainning, our data show that dexamethasone inhibited cell proliferation and an increased number in the dentate gyrus of rats after acupuncture has been observed. The phenotype of BrdU positive cells were examined by double-labeling with the neuronal marker-NeuN, and astrocyte marker-GFAP. The percentages of cells colocalized with BrdU and NeuN in each group shows no virtually difference. The results will provide solid evidence to support that acupuncture treatment can improve the glucocorticoid damaged neuronal tissue. However the differentiation of precursor cells into neuron or glia cell needs further investigation.
| Identifer | oai:union.ndltd.org:TW/093NCKU5391008 |
| Date | January 2005 |
| Creators | Hung-Nan Wang, 汪宏南 |
| Contributors | Chi-hsien Chien, Yung-hsien Chang, 簡基憲, 張永賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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