碩士 / 國立東華大學 / 生物技術研究所 / 93 / When euryhaline tilapia is transferred from freshwater (FW) to seawater (SW), the osmolarity response of body including gill and brain makes an attempt to regulate ions balance. However, the involvement of stress activated protein kinase signaling pathways in the osmoregulation of tilapia brain during salinity changes is still unclear. The present study was conducted to examine the alterations of MAP Kinase, PI-3 Kinase and NF-κB pathways of tilapia (Oreochromis mossambicus) brain in response to SW (25 ppt or 35 ppt) transfer. Total protein of tilapia brain after SW transfer at various times was isolated and applied for western blotting. After SW transfer, the osmolarity of tilapia brain is acutely elevated and the higher osmolarity change of brain appears at the higher salinity level. The protein levels of Ras/ERK, PI-3 Kinase and NF-κB pathways are gradually increased after 25 ppt SW transfer while the protein levels of JNK, p38 Kinase and PI-3 Kinase pathways are gradually increased after 35 ppt SW transfer. The increment of Caspase 3 protein after 35 ppt SW transfer is greater than that of 25 ppt SW transfer. The incidence of apoptosis in tilapia brain after 35 ppt SW transfer is more severe than that after 25 ppt SW transfer, this may be the reason why tilapia is dead after 35 ppt SW transfer. These results suggest that the osmolarity change of tilapia brain might affect the expressions of MAP Kinase, PI-3 Kinase and NF-κB pathways within 4 h after 25 ppt SW transfer and make tissues and cells resistance to apoptosis. Finally, the β-actin protein is increased after 35 ppt SW transfer, the data imply that the higher osmolarity leading to cell cytoskeleton change needs to further study.
| Identifer | oai:union.ndltd.org:TW/093NDHU5108017 |
| Date | January 2005 |
| Creators | Pei-Lin Wu, 吳佩霖 |
| Contributors | C.-F. Weng, 翁慶豐 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 101 |
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