碩士 / 國立屏東科技大學 / 水產養殖系 / 93 / LGBP cDNA was cloned from the haemocytes and hepatopancreas of white shrimp Litopenaeus vannamei by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers. Analysis of nucleotide sequence revealed that the cDNA clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. The calculated molecular mass of the mature proteins is 39.92 kDa with an estimated pI of 4.37. Two putative integrin binding motifs (cell adhesion site), RGD (Arg-Gly-Asp) and a potential recognition motif for β-(1 → 3) linkage of polysaccharides were observed in the LGBP. Sequence comparison showed that LGBP deduced amino acid of L. vannamei has an overall similarity of 95%, 92% and 61% to that of blue shrimp Litopenaeus stylirostris LGBP, tiger shrimp Penaeus monodon BGBP and crayfish Pacifastacus leniusculus LGBP, respectively. They are with conserved features including a potential polysaccharide-binding motif, a glucanase motif, and N-glycosylation sites. To evaluate exogenous and endogenous factors that affect the expressions of LGBP genes, quantitative real-time PCR was conducted. The results were as follows: During different molting stages, LGBP transcripts in haemocytes at D2/3 stage were significantly higher than that at C stage, while the LGBP transcripts expression in hepatopancreas at the C stage is the highest. LGBP transcripts in haemocytes were up-regulated significantly after 3 and 6 h post Vibrio alginolyticus-injections, but the LGBP transcripts in hepatopancreas showed no significant differences. After feeding the diet containing 1 g kg-1 sodium alginate for a week, the expressions of LGBP transcripts in hepatopancreas were significantly lower than that of control groups, while the LGBP transcripts expressions in haemocytes showed no significant differences. The LGBP transcripts expressions in haemocytes at 18 and 34 groups were significantly higher than 26 ℃group after 48h thermal treatment, while the LGBP transcripts profiles in hepatopancreas at 18 and 26 ℃groups were significantly higher than 34 ℃ group after 168 h thermal treatment. The LGBP transcripts expressions in hepatopancreas in salinity 25 ‰ were significantly higher than that in 30 ‰, while LGBP transcripts in haemocytes showed no significant differences. The LGBP transcripts expressions in hepatopancreas were increased significantly while shrimps immersed in 10 mg/l ammonia-N after 48 h, but the LGBP transcripts expression in haemocytes showed no significant differences. The LGBP transcripts in haemocytes were increased significantly while shrimps immersed in 10 mg /l nitrite-N after 48 h, and 5 and 10 mg/l nitrite-N after 168 h, while the LGBP transcripts expressions in hepatopancreas treated in 5 mg/l nitrite-N after 48 h were increased significantly.
| Identifer | oai:union.ndltd.org:TW/093NPUST086006 |
| Date | January 2005 |
| Creators | Chiung-Hui Tsai, 蔡瓊慧 |
| Contributors | 鄭文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 109 |
Page generated in 0.0016 seconds