碩士 / 國立中山大學 / 海洋資源學系研究所 / 93 / The central 5-hydroxytryptamine ( Serotonin, 5-HT ) system play an
important role in the brain sexual differentiation in tilapia,Oreochromis
mossambicus. The 5-HT1A receptor is involved in the neural develop-
ment of brain. The cDNA sequences of 5-HT1A receptor of tilapia, Ore-
ochromis mossambicus have been cloned in our laboratory. In the
present study, 1633 bp of DNA sequence of 5-HT1A receptor from the
transcription start site ( TSS ) were cloned by 5’ rapid amplification of
cDNA end ( 5’ RACE ) and Genome Walker DNA Walking. The 1633
bp of DNA sequence divided into six different fragments to detect the
luciferase activity in the HeLa cell. The bioinformatic analysis was
performed on the fragment which showed the highest luciferase activity
for predicting the transcription factor binding site. These results showed
that there is a activator protein-1 ( AP-1 ) binding site in the fragment
form -52 bp to -46 bp. The site-direct mutagenesis and electrophoresis
mobility shift assay was performed on the fragment form -52 bp to -46 bp
. The present results indicate that there is a transcription factor binding
site in the fragment form -52 bp to -46 bp. This transcription factor
binding site could be a AP-1 binding site.
| Identifer | oai:union.ndltd.org:TW/093NSYS5277008 |
| Date | January 2005 |
| Creators | Tsai-jia shiun, 蔡佳訓 |
| Contributors | Ching-Lin Tasi, 蔡錦鈴 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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