博士 / 國立清華大學 / 生命科學系 / 93 / Cytochrome P450 is a superfamily of heme containing monooxygenase that catalyze the oxidative and reductive metabolism of many drugs, environmental chemicals, and endogenous compound. On the basis of sequence similarity, the CYPs can be classified into various families and subfamilies.
Here I have cloned seven CYP genes by rapid amplification of cDNA ends (RACE) approach. On the basis of nucleotide and amino acid sequences, the identified cDNAs was assigned as CYP3A65, CYP1C1, CYP2K8, CYP2AD3, CYP2V1, CYP2X10 and CYP2X12 by the Cytochrome P450 Nomenclature Committee. CYP genes expression pattern in zebrafish embryo was investigated by whole mount RNA in situ hybridization. The CYP3A65 mRNA is initially transcribed only in the liver and gut after hatching. In contrast the CYP1C1 mRNA is expresse in blood vessel. Weak transcription of CYP1C1 is still detectable in larval vascular system. The CYP2K8 mRNA is expressed in the branchial arch before hatching. After hatching the CYP2K8 is mainly expressed in the liver and gut. The CYP2AD3 mRNA is expressed in pia mater, branchial arch, liver and gut. The CYP2V1 mRNA is expressed in olfactory bulb, branchial arch, liver and gut. The CYP2X10 mRNA is expressed in the brain, liver and gut. The CYP2X12 mRNA is expressed in the brain, liver and gut.
During the larval stages, CYP 3A65 transcription in the gut is greatly enhanced by steroid dexamethasone, macrocyclic antibiotic rifampicin or TCDD exposure. CYP1C1 transcription is also enhanced by dexamethasone and TCDD treatment.
Repression of AhR2 translation by antisense morpholino oligonucleotides abrogated both of constitutive and TCDD, dexamethasone or rifampicin-stimulated CYP3A65 transcription in larval intestine. Similar abrogation of constitutive and drug-induced CYP1C1 transcription is also observed in AhR2-morpholino injected embryo.
| Identifer | oai:union.ndltd.org:TW/093NTHU5105011 |
| Date | January 2005 |
| Creators | 曾華斌 |
| Contributors | , 許宗雄, 胡清華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 111 |
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