碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / The processes and purposes of this study were first to use zeolite as a carrier to enrich Artemia nauplii and copepod through Experiment I and II, respectively, with astaxanthin (AX) and canthaxanthin (CN) and find out the effects of such enrichment on growth, survival, and body carotenoid enhancement of the resulting prey organisms. Experiment III was to feed these enriched prey organisms to postlarvae (PL) of Pacific white shrimp (Litopenaeus vannamei) and black tiger prawn (Penaeus monodon) to find out the effects of these enriched prey organisms on body carotenoid enhancement of the resulting shrimp PL. These PL were then exposed to low dissolved oxygen (DO) stress (initial DO = 0.5 mg/L) for 1 h to find out the effects of enrichment of different carotenoid (AX or CN) and resulting prey organisms (Artemia or copepod) on stress resistance of the resulting fry.
In Experiment I, Artemia nauplii 6 days-post-hatch were fed zeolite containing AX or CN at 6 concentrations for 1 day (D2) then fed carotenoid-free zeolite for another day (D3). More than 90% of the body carotenoid of the Artemia fed with AX containing zeolite (AX Artemia) was CN on D2, showing that Artemia could convert AX to CN. The progressive loss of body CN content, even 64% in a day, suggested enriching body CN in Artemia would be necessary. Dietary AX was 59% more efficient than dietary CN in retaining body CN content in Artemia. Positive correlations of dry weight of the sampled Artemia versus zeolite AX or CN concentration, body CN content of AX Artemia or CN Artemia, and body AX content of AX Artemia all indicated the significance of AX or CN in dietary nutrition of Artemia. In Experiment II, copepod were fed same diet (zeolite) regime as Experiment I. Dietary AX was more efficient than dietary CN in enhancing and retaining body total astaxanthin (TA) content in copepod on D2 and D3. Positive correlations of dry weight of the sampled copepod versus zeolite AX or CN concentration, body AX content of AX copepod or CN copepod all indicated the significance of AX or CN in dietary nutrition of copepod.
In Experiment III, after 24-h enrichment, Artemia had higher body carotenoid (mainly CN) than copepod (all AX). After the low DO stress, for black tiger prawn, those PL which had fed copepod had higher survival rate than those had fed Artemia. However, for Pacific white shrimp, there was no difference in survival rate between these two groups. A correlation matrix showed that monoester AX, diester AX, free AX, and total AX in PL of both shrimp were positively affected by the AX in prey, but not CN. PL which had fed prey having high body AX had high antioxidant status, namely, high total antioxidant status and glutathione reductase and low superoxide dismutase and thiobarbituric acid reactive substances, concomitantly, high resistance to low DO stress and high survival rate. In conclusion, the results of this study suggest that prey organisms enriched with AX is favorable to the predator PL in resisting low DO stress.
| Identifer | oai:union.ndltd.org:TW/093NTOU5086004 |
| Date | January 2005 |
| Creators | Chien-Chung Lee, 李建忠 |
| Contributors | Yew-Hu Chien, 陳瑤湖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 137 |
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