碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Abstract
A study was conducted on the indoor culture of Gelidium amansii for seedling source, and the growth and development of the seedling attached on artificial substrate.
G. amansii suspending culture in tank for 3 month will gradually change their pinnate fan-shape erect thalli into a ball-like shape, with new born round branches arising from every direction.
In the experiment of seedling culture(in suspending), 20℃ in the best for growth in 4 week(179.46±10.82%) as compared to the other two temperature(15℃ and 25℃). The enrichment of N between 0~200µM exhibit no significant effect on growth whereas the addition of phosphate effectively raised the growth rate as compared to that in seawater.
After attachment the seedlings were found to regenerate new branches on the cut surface or somewhere else in opposite or alternative way, which creeping on the substratum surface.
Artificial substrate(sub2) made from 50% fly ash+50% coal ash was selected as the substrate in the subsequent experiment since it exhibit the closest to those on natural substrates(coral sheet), including the values of new born branch number, attachment point and residual attachment ratio.
In a porosity experiment with sub2, the one(sub8) with 41.57 ±0.3% porosity was found to be better than that with 56.72%±0.81%(sub9). Thus the sub8 was used in the subsequent growth and development experiment.
The growth of the branches attached on artificial substrates were similar to those obtained from suspending culture, 20℃ showed the best effect on linear growth on substrate. In 12 days of growth, it reached a value of 190.33±8.47.Nutrition enrichment experiment also came to a similar conclusion. The addition of 20µM nitrate may be the best treatment in a 15 day experiment.
In the light effect experiment, it was found that seedling growth would increase with the raising of light intensity as well as the length of photoperiod in the first two weeks. However, extent of bleaching also affected by the two light factor in a similar manner. The appearance of erect-flatten pinnae on the creeping thalli also showed the same trend in response to light as described for the initial growth of seedling on artificial substrate. The erect pinnae, however, only grew to 0.5cm high while a round branchlet raised from their top to reattach to the substrate surface. The expression of highest specific growth rate, lowest bleaching rate and highest potential in the appearance of erect pinnae led to a conclusion that the combination of light intensity at 300µEm-2s-1 and light regime 8L:16D would be the best light condition for resuming the natural habit in G. amansii.
| Identifer | oai:union.ndltd.org:TW/093NTOU5086005 |
| Date | January 2005 |
| Creators | Min-Yao Lin, 林銘曜 |
| Contributors | 陳忠信 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 107 |
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