碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 93 / Teleost fishes are persistent hyperglycemia after ingesting the fodder with high concentration carbohydrate, so teleost fishes are thought to be unable to utilize carbohydrate effectively. The purpose of this thesis is mainly to study the mRNA expression levels of glycolytic genes in Tilapia liver by different time of glucose induced with quantitative real-time polymerase chain reaction ( Real-time PCR ) technology. These measured genes include the enzymes in glycolysis pathway , Phosphoglucomutase ( Glycogenesis pathway ) , Glucose-6-phosphate dehydrogenase ( Pentose phosphate pathway ) , and Glucose-6- phosphatase ( gluconeogenesis pathway ). We hope understand the glycolytic pathway proceeded by significant variations of the glycolytic gene expression levels between different time of glucose induced when glucose into cell. In result , get the partial sequences of those analysis genes by degenerate primers design and RT-PCR technology , and Real-time PCR was used to determine the mRNA expression level of those glycolytic genes in Tilapia liver by glucose induced ( 0 hr to 18 hrs ). When isolated the mRNA of Tilapia liver by glucose induced were studied , the mRNA levels of Hexokinase , Phosophoglucoisomerase and Phosophofrutokinase increased significantly after glucose induced 0 hr to 18hrs. Glyceraldehyde 3- phosphate dehydrogenase and Phosphoglycerate kinase mRNA levels remained unchanged after glucose induced 0 hr to 18hrs. In contrast, the mRNA levels of Glucose-6-phosphate dehydrogenase , Phosphoglucomutase and Glucose-6-phosphatase decreased significantly after glucose induced 0 h to 18h. Interesting , Triose phosphate isomerase and Phosphoglycerate mutase mRNA levels also decreased significantly after glucose induced 0 h to 18h. In addition , we focus some specific genes to determine the mRNA expression level of those glycolytic genes in Tilapia liver by short time of glucose induced ( 0 hr to 6 hrs ) , These measured genes include Hexokinase , Phosophofrutokinase , Triose phosphate isomerase , Phosphoglycerate mutase, Glucose-6-phosphate dehydrogenase , Phosphoglucomutase and Glucose-6-phosphatase. The measured result of those genes expression like the result of the longer time glucose induced ( 0 hr to 18 hrs ). So the gene expression of Triose phosphate isomerase and Phosphoglycerate mutase maybe hinder the glycolysis pathway that go on, and the measured result of Glucokinase and Glucose-6-phosphate dehydrogenase not like the result of other references. These genes worth going for discussion further in the future.
| Identifer | oai:union.ndltd.org:TW/093NTOU5086029 |
| Date | January 2005 |
| Creators | Bo-Yan Jheng, 鄭博元 |
| Contributors | Jau-Der Chen, 陳昭德 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 252 |
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