碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 93 / This thesis discusses the community structure of phytoplankton was investigated from pigment composition of East China Sea during June 2003 to July 2004 on three summer cruises. Pigment concentration determination by high-performance liquid chromatography was interpreted using a matrix fastorization program “CHEMTAX” to obtain the Chlorophyll a biomass of phytoplankton groups at class level.
As for the northwestern part of the East China Sea, Especially the near-coastal of China Mainland, Chlorophyll a concentration were enriched all year-round, whilst the OR1-686(June, 2003) has the most highest abundance, followed by OR1-721(June, 2004) and OR1-691(August, 2004). The results indicating this might be the influence of the Changjiang fresh water discharge.
As a whole, the dominant phytoplankton near China Mainland coastal presented notable difference in these three cruises, cause of the hydrological factor in this area. However, we discovered that bacillariaphyceae, dinophytes and cryptophytes have high contribution in the nutrient-rich region. While the off-shelf region, chrysophytes were the most abundant taxa followed by prasinophytes, cyanobacteria and prochlorophytes.
Hence HPLC determination of the biomarker pigment is a useful tool for quantitative of each phytoplankton groups. At present, combined with CHEMTAX will be the most robust way to analyze dynamic of phytoplankton populations.
Over 50 % of photosynthesis were carried on picophytoplankton in the ocean, therefore they play an important role for the primary production and the global carbon cycling in the ocean. These tiny(2 μM), unicellular picophytoplankton were included Prochlorococcus and Synechococcus. The purpose of this thesis is to investigate the distribution and the contribution of picophytoplankton in the East China Sea near by Taiwan coastal.
Water samples were collected in August 2004 during the OR2-1213 cruise. Then we count the number of cells by Flow Cytometry, using HPLC to analyze the pigments and to determine the 16S rDNA sequences of these picophytoplankton. The results showed prochlorococcus occured only at the open ocean, and the average contribution were 0.112 μg L-1 ; the synechococcus have a ubiquitous distribution, and have a higher average contribution (0.291 μg L-1)at the continent shelf, where the open ocean were 0.124 μg L-1 .
We found the microdiversity of picophytoplankton in East China Sea, and suggested that the picophytoplankton genetic bases were evolution to different kinds, due to the biogeographical boundary in this site.
| Identifer | oai:union.ndltd.org:TW/093NTOU5111007 |
| Date | January 2005 |
| Creators | Wai-Mei Ng, 吳慧敏 |
| Contributors | Tung-Ming Hsiung, 熊同銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 171 |
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