博士 / 國立臺灣海洋大學 / 食品科學系 / 93 / Abstract
The purpose of the study was to estimate vitamin C requirements of juvenile grouper, Epinephelus malabaricus by using L-ascorbic acid (AA) and its four derivatives namely, L-ascorbyl-2-monophosphate-Mg (C2MP-Mg), L-ascorbyl-2-monophosphate-Na (C2MP-Na), L-ascorbyl- 2-sulfate (C2S) and L-ascorbyl-2-polyphosphate (C2PP). The effects of these vitamin C sources on stress and immune responses in grouper were also monitored. The requirement established was 45.3 mg AA/kg diet from AA; 17.9 and 8.3 mg AA/kg diet from C2MP-Mg and C2MP-Na, respectively; 46.2 and 17.8 mg AA/kg diet from C2S and C2PP, respectively; 10.3 and 12.8 mg AA/kg diet from C2MP-Na and C2PP, respectively. It indicates that C2MP-Mg, C2S and C2PP is 46%, 39% and 81% as effective as C2MP-Na, C2PP and C2MP-Na, respectively in meeting the vitamin C requirement for grouper. Overall, the efficiency of AA and the four derivatives as vitamin C source in grouper are in the order of C2MP-Na＞C2PP＞C2MP-Mg＞AA＞C2S. The data also revealed that both AA and the derivatives support the non-specific immune responses, including O2- production ratio, alternative complement activity and lysozyme activity, of the fish. Six times of the adequate vitamin C requirement from AA is needed to enhance the non-specific immune responses and maintain survival of the fish challenged with the bacteria Vibrio carchariae. Exposure to low temperature suppressed lysozyme activity and O2- production ratio in fish challenged with V. carchariae. At least five times of the adequate vitamin C requirement from C2MP-Na or C2PP is needed to minimize the suppression of immune responses in fish caused by low temperature stress. Exogenous adrenocoticotropic hormone (ACTH) injection resulted in cortisol increase in plasma and AA depletion in interrenal tissue of the fish. Ten times of the adequate vitamin C requirement from C2MP-Na or C2PP is required to regulate cortisol biogenesis in grouper. C2PP exhibited a vitamin E sparing effect in fish. Lysozyme activity and alternative complement activity in fish were synergistically enhanced by the supplementation of C2PP and vitamin E.
| Identifer | oai:union.ndltd.org:TW/093NTOU5253002 |
| Date | January 2005 |
| Creators | Mei-Fang Lin, 林美芳 |
| Contributors | Shi-Yen Shiau, 蕭錫延 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 183 |
Page generated in 0.0019 seconds