博士 / 國立臺灣海洋大學 / 食品科學系 / 93 / In ten algal polysaccharides, the algal polysaccharide extracts (APEs) of Porphyra (Por.) dentate possess greatest effects on five kinds antioxidative and four types antimutagenic experiment. And the antioxidative properties and antimutagenicity of ten algal polysaccharides were related to their polyphenol contents. The ten algal polysaccharides had not observed the ability on the ACE inhibitory. On six antioxidative experiment, the 120 algal-oligosaccharide- lysates (AOLs) had scavenging abilities, except for hydroxyl radicals scavenging effect. Without the addition of S9 mixtrues in Ames test, the antimutagenicity of 60 AOLs against 4NQO to Salm. typhimurium TA98 or TA100 were showed from12-94% or 4-98%, respectively. With the addition of S9 mixtrues in Ames test, the antimutagenicity of 60 AOLs against B[a]P to Salm. typhimurium TA98 or TA100 were reveal from 18-89% or 24-99%, respectively. The effect of antioxidative properties and antimutagenicity of 120 AOLs were relations with their polyphenol contents. The AOL derived from APEs of Mon. nitidum digested by B500 treatment had 17.1 �b 1.2% inhibitory of ACE was better than other AOL.
The algal-oligosaccharides solution (108-Por500) derived from3 L of 0.5% APEs of Por. dentate digested by 15,000 AU of MAEF108-agarases were categorized as following 5 fractions: (i) > 5 KDa agar-lytic, (ii) 5-3 KDa agar-lytic, (iii) 3-1 KDa agar-lytic, (iv) < 1 KDa agar-lytic and (v) polyphenolic fraction. The (i)-(iv) agar-lytic fractions without exist polyphenol contents, and the (v) polyphenolic fraction exist polyphenol. The neoagarooligosaccharides, such as neoagarobiose, neoagarotetraose, and neoagarohexaose colud be able to separate from 1 KDa agar-lytic fraction. The (i)-(iv) agar-lytic fractions displayed 2.48-17.85% on chelating effect in ferrous ion, and whitout antioxidative abilities on the other five antioxidative tests. On the other hand, the polyphenolic fraction exist scavenging abilities on six antioxidative properties. In four types antimutagenic experiment, the polyphenolic fraction present antimutagenicity were greater than 90%.
The 90 lactic acid fermentation solutions derive from (1) algal polysaccharide lactic acid fermentation substrate (APsLAFSs), (2) algal oligosaccharide lactic acid fermentation substrate (AOsLAFSs), or (3) algal oligosaccharide plus galactose lactic acid fermentation substrate (AOsGLAFSs) which fermented by Streptococcus (Strep.) faecalis BCRC13076 and Lactobacillus (Lact.) plantarum BCRC1406 could decreased pH value below 4.6 within 24 hr at 37oC. During storage at 4oC, 90 lactic acid fermentation solutions, exhibited decrease pH, increase TA, decrease viability, and an increasing reduction in sugar contents from 1 to 2 weeks. In the 90 lactic acid fermentation products, except for DPPH radical scavenging effect, the other five kinds antioxidative tests had effect increasing with reducing sugar content. Without the addition of S9 mixtrues in Ames test, the antimutagenicity of 90 lactic acid fermentation products against 4NQO to Salm. typhimurium TA98 or TA100 were ranged from 26-99% or 33-97%, respectively. With the addition of S9 mixtrues in Ames test, the antimutagenicity of 90 lactic acid fermentation products against B[a]P to Salm. typhimurium TA98 or TA100 were exhibited from 4-98% or 33-99%, respectively. The ACE inhibitory of AOsGLAFSs-Mon was 36.6 �b 6.6% and better than other lactic acid fermentation products.
The 30 acetic acid fermentation solutions derive from (1) algal polysaccharide acetic acid fermentation substrate (APsAAFSs), (2) algal oligosaccharide acetic acid fermentation substrate (AOsAAFSs), or (3) algal oligosaccharide plus galactose acetic acid fermentation substrate (AOsGAAFSs) which fermented by Acetobacter (Acet.) pasteurianus BCRC11070 could increased TA above 1% within 48 hr at 26oC. During storage at 4oC, 90 lactic acid fermentation solutions, exhibited decrease pH, increase TA, decrease viability, and an increasing reduction in sugar contents from 1 to 2 weeks. In the 30 acetic acid fermentation products, except for without the hydroxyl raducals scavenging effect, other five kinds antioxidative tests had scavenging effect increasing with reducing sugar content. Without the addition of S9 mixtrues in Ames test, the antimutagenicity of 30 acetic acid fermentation products against 4NQO to Salm. typhimurium TA98 or TA100 were ranged from 2-56% or 3-76%, respectively. With the addition of S9 mixtrues in Ames test, the antimutagenicity of 30 acetic acid fermentation products against B[a]P to Salm. typhimurium TA98 or TA100 were revealed from 6-88% or 3-75%, respectively. The ACE inhibitory of 30 acetic acid fermentation products were revealed 3.4 �b 0.7% to 53.9 �b 3.7%
| Identifer | oai:union.ndltd.org:TW/093NTOU5253069 |
| Date | January 2005 |
| Creators | Shao-Chi Wu, 吳紹祺 |
| Contributors | Chorng-Liang Pan, 潘崇良 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 335 |
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