碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 93 / Abstract
In most vertebrate animals, the development of the immature oocyte into a fertilizable gamete, a process known as oocyte maturation, involves an arrest in the meiotic cell cycle while awaiting fertilization. Mos, a serine/threonine kinase, is specifically expressed during meiotic maturation of vertebrate oocytes. After germinal vesicle breakdown (GVBD), Mos is involved in the regulation of meiotic spindle assembly and chromatin organization. The activation of this kinase is also essential for the maintenance of metaphase Ⅱ arrest.
Ran GTPase, which belongs to the Ras superfamily of small GTPase, and the protein that regulate its GTP binding and hydrolysis has a well-defined role in nuclear transport. Recent studies indicate that Ran has a central role in spindle assembly and nuclear envelope reformation. It seems that Ran system and Mos pathway coordinate in some events in M phase. To understand the inter-relationship of them, I ectopically express Carp Mos gene in COS-1 cell and investigate Mos-interacting proteins. Heat Shock Protein-Hsp70 and Hsp90 are detected in Mos co-immunoprecipitation complex with or without kinase activity, but Ran dose not exist in the complex.
| Identifer | oai:union.ndltd.org:TW/093NTU05061002 |
| Date | January 2005 |
| Creators | Kuan-Cheng Lee, 李冠徵 |
| Contributors | 柯逢春 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
Page generated in 0.0022 seconds