碩士 / 國立臺灣大學 / 分子與細胞生物學研究所 / 93 / Troponin I (TnI), a troponin component, inhibits the generation of acto-myosin and controls muscle contraction. Cardiac TnI is highly sensitive and specific markers of myocardial injury in acute coronary syndromes. Although troponin I genes have been identified in birds and mammals that encode the isoforms expressed in cardiac muscle, slow skeletal muscle and fast skeletal muscle, there is extremely little known about troponin I genes in lower vertebrates. In order to analyze the molecular structure and dynamic expression of TnI gene in lower vertebrates, we cloned and characterized the zebrafish cTnI (cardiac TnI), sTnI (slow TnI ), and fTnI (fast TnI ). The deduced amino acid sequences of zebrafish cTnl, sTnl and fTnl shared 62%, 50% and 43% identities with human cTnI, sTnI and fTnI, respectively. RT-PCR showed that zebrafish cTnI, sTnI and fTnI mRNA were inherited in a maternal manner. Whole-mount in situ hybridization revealed that the zebrafish cTnI was cardiac-muscle specific, sTnI was slow-muscle specific and fTnI was fast-muscle specific. However, zebrafish sTnI signal disappeared in the somite at 50 hours-post-fertilization (hpf) embryos, while fTnI signal disappeared at 54 hpf embryos. The expression of zebrafish sTnI was in the fin bud at 72 hpf embryos; whereas fTnI was expressed in the fin bud, sternohyoideus and adductor mandibulae cranial muscles at 72 hpf embryos. These findings may provide us new insight about developmental regulation and molecular evolution of vertebrate TnI isofroms.
| Identifer | oai:union.ndltd.org:TW/093NTU05061018 |
| Date | January 2005 |
| Creators | Chen-Min Lin, 林晨敏 |
| Contributors | 蔡懷楨 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 57 |
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